Abstract
Optimization and validation of a capillary zone electrophoresis (CZE) method for the determination of inner and external inorganic sulfate from doxorubicin-loaded liposomes as a measurement of encapsulation efficiency are presented. Sulfate was determined using a fused silica capillary (50 cm x 75 μm ID) and background electrolyte (BGE) composed of 5 mM chromate with 1 mM of TTAB at pH 10.5 with indirect UV detection at 254 nm. The separations were achieved at temperature of 25 ºC, 5 kV of reverse applied voltage using a pressure of 0.5 psi for 5s of sample introduction. Sulfate quantitation from the outside of liposomes was obtained by simple dilution of the liposome sample but an extraction with a non-ionic surfactant and a mixture of methanol-chloroform-water were necessary to breakdown the liposomes for the quantitation of the total sulfate (inside and outside) with acceptable recovery percentages (96.3% to 102.6%). Parameters of validation were evaluated according to ICH guidelines such as specificity, linearity, LOD, LOQ, accuracy, precision and robustness. The proposed CE method resulted to be suitable for the inner and external sulfate analysis of doxorubicin-loaded liposomes for routine quality and stability control studies.
Keywords: Doxorubicin encapsulation, liposomes, sulfate, capillary zone electrophoresis.
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
Related Journals
Anti-Cancer Agents in Medicinal Chemistry
Current Computer-Aided Drug Design
Current Bioactive Compounds
Combinatorial Chemistry & High Throughput Screening
Current Medicinal Chemistry
The Natural Products Journal
Current Catalysis
Current Chinese Chemistry
Current Chinese Science
Current Topics in Chemistry
Related Books
Botanicals and Natural Bioactives: Prevention and Treatment of Diseases
Biosurfactants: A Boon to Healthcare, Agriculture & Environmental Sustainability
Frontiers in Computational Chemistry
Advances in Dye Degradation
Biological and Medical Significance of Chemical Elements
Frontiers In Medicinal Chemistry
Advances in Organic Synthesis
Frontiers in Natural Product Chemistry
Recent Advances in Analytical Techniques
Advanced Catalysts Based on Metal-organic Frameworks (Part 2)
22