Abstract
A rapid and simple microemulsion electrokinetic chromatography (MEEKC) method has been developed and validated for the determination of ethinylestradiol and levo-norgestrel in a commercial tablet formulation. The analysis was performed with an electrolyte composed of 0.5% (w/w) ethyl acetate, 1.2% (w/w) butan-1-ol, 0.6% (w/w) sodium dodecyl sulfate, 15% (v/v) ethanol and 82.7% (w/w) 12 mmol L−1 sodium tetraborate aqueous buffer at pH 9.23 and direct UV detection at 200 and 247 nm. For quantitative purposes, estrone was used as an internal standard. Retention time for levo-norgestrel and ethinylestradiol was approximately 2.1 and 2.5 min respectively. Acceptable precision (<0.90% RSD, linearity (r2 > 0.99; concentration range from 24.0 to 36.0 µg mL−1), limits of detection (LOD) and quatification (LOQ) for ethinylestradiol of 1.0 μg mL-1 and 3.2 µg mL−1, respectively, and for levo-norgestrel of 1.4 µg mL−1 and 4.1 µg mL-1, respectively, and recovery of 100 ± 2 % at three concentration levels were obtained. Based on the performance characteristics, the proposed methodology was found suitable for the determination of ethinylestradiol and levo-norgestrel in tablet formulations.
Keywords: Contraceptives, capillary electrophoresis, ethinylestradiol, levo-norgestrel, Microemulsion electrokinetic chromatography, steroids.
Current Analytical Chemistry
Title:Determination of Ethinylestradiol and Levo-Norgestrel Using Microemulsion Electrokinetic Chromatography
Volume: 10 Issue: 2
Author(s): Claudinei Alves da Silva, María Segunda Aurora-Prado and Marina Franco Maggi Tavares
Affiliation:
Keywords: Contraceptives, capillary electrophoresis, ethinylestradiol, levo-norgestrel, Microemulsion electrokinetic chromatography, steroids.
Abstract: A rapid and simple microemulsion electrokinetic chromatography (MEEKC) method has been developed and validated for the determination of ethinylestradiol and levo-norgestrel in a commercial tablet formulation. The analysis was performed with an electrolyte composed of 0.5% (w/w) ethyl acetate, 1.2% (w/w) butan-1-ol, 0.6% (w/w) sodium dodecyl sulfate, 15% (v/v) ethanol and 82.7% (w/w) 12 mmol L−1 sodium tetraborate aqueous buffer at pH 9.23 and direct UV detection at 200 and 247 nm. For quantitative purposes, estrone was used as an internal standard. Retention time for levo-norgestrel and ethinylestradiol was approximately 2.1 and 2.5 min respectively. Acceptable precision (<0.90% RSD, linearity (r2 > 0.99; concentration range from 24.0 to 36.0 µg mL−1), limits of detection (LOD) and quatification (LOQ) for ethinylestradiol of 1.0 μg mL-1 and 3.2 µg mL−1, respectively, and for levo-norgestrel of 1.4 µg mL−1 and 4.1 µg mL-1, respectively, and recovery of 100 ± 2 % at three concentration levels were obtained. Based on the performance characteristics, the proposed methodology was found suitable for the determination of ethinylestradiol and levo-norgestrel in tablet formulations.
Export Options
About this article
Cite this article as:
Silva Alves da Claudinei, Aurora-Prado Segunda María and Tavares Franco Maggi Marina, Determination of Ethinylestradiol and Levo-Norgestrel Using Microemulsion Electrokinetic Chromatography, Current Analytical Chemistry 2014; 10 (2) . https://dx.doi.org/10.2174/15734110113099990007
DOI https://dx.doi.org/10.2174/15734110113099990007 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
Biogeochemistry: global environmental research and management
his issue focuses on the bio-pathways of trace elements (Ni, Co, Cr, Mn, Cd, Pb, Zn and REE) in soil, plant and atmospheric systems. Specific interest in the application of phytotechnologies that utilise hyperaccumulator plants (agro/phytomining). On the basis of understanding the migration and transformation of trace elements in ecosystem, ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
The Therapeutic Properties of Carapa guianensis
Current Pharmaceutical Design Application of Liposomes in Cancer Therapy: An Assessment of the Advancement of Technology Through Patent Documents
Recent Patents on Nanotechnology Electrocatalytic Determination of 6-Mercaptopurine Using Multiwall Carbon Nanotubes Paste Electrode in the Presence of Methyldopa
Current Nanoscience Disease Progression in HIV Late Presenters: the Role of HIV Clinical Indicator Diseases Prior to HIV Diagnosis
Current HIV Research Deciphering the Antibodyome - Peptide Arrays for Serum Antibody Biomarker Diagnostics
Current Proteomics Vascular Arginase and Hypertension
Current Hypertension Reviews Mechanisms and Treatment of Neuropathic Pain
Central Nervous System Agents in Medicinal Chemistry Application of Microdialysis in Clinical Pharmacology
Current Clinical Pharmacology Synthesis, Aromatase Inhibitory, Antiproliferative and Molecular Modeling Studies of Functionally Diverse D-Ring Pregnenolone Pyrazoles
Anti-Cancer Agents in Medicinal Chemistry Neuroprotective Appraisal of Methanolic Extract of Flowers of Nerium oleander in a Non Classical Rat Model of Alzheimer Disease
The Natural Products Journal Identify DNA-Binding Proteins with Optimal Chou’s Amino Acid Composition
Protein & Peptide Letters Interactions of Cisplatin with non-DNA Targets and their Influence on Anticancer Activity and Drug Toxicity: The Complex World of the Platinum Complex
Current Cancer Drug Targets Novel 1,3,4-Thiadiazole Linked Amide Derivatives of Pteridone: Synthesis and Study of Anticancer Activities
Letters in Organic Chemistry An Efficient, Flexible-Model Program For The Analysis Of Differential Scanning Calorimetry Protein Denaturation Data
Protein & Peptide Letters Equinatoxin II Potentiates Temozolomide- and Etoposide-Induced Glioblastoma Cell Death
Current Topics in Medicinal Chemistry Recent Advances in Understanding Hormonal Therapy Resistant Prostate Cancer
Current Cancer Drug Targets Gadolinium Oxide Nanoparticles as Potential Multimodal Imaging and Therapeutic Agents
Current Topics in Medicinal Chemistry Prediction of Acridinones’ Ability to Interstrand DNA Crosslinks Formation Using Connected QSRR and QSAR Analysis
Letters in Drug Design & Discovery HR-MAS NMR Spectroscopy: A Practical Guide for Natural Samples
Current Organic Chemistry A3 Receptor Ligands: Past, Present and Future Trends
Current Topics in Medicinal Chemistry