Qi-Shao-Shuang-Gan Prevents Adhesion between Leukocytes and Inflammatory Endothelial Cells
Xinghua Gao, Difei Bian, Xianxiang Xu, Rong Pan, Yufeng Xia, Side Jiang and Yue Dai
Pages 165-172 (8)
Our previous studies demonstrated that Qi-Shao-Shuang-Gan (QSSG), a combination of Astragalus
membranaceus saponins (SAM) with Paeonia lactiflora glycosides (GPL), possessed protective effects against sepsis in
mice. The present study aimed to explore its underlying mechanisms with a focus on the adhesion between leukocytes and
inflammatory endothelial cells. Our results showed that QSSG (10, 30, 100 μg/ml) concentration-dependently inhibited
the adhesion between leukocytes and lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells
(HUVECs), and downregulated the expressions of endothelial adhesion molecules such as E-selectin, P-selectin,
intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in HUVECs. It also
suppresssed the phosphorylations of JNK, ERK and p38 MAPK, as well as the activation of NF-κB in HUVECs. The
findings revealed that QSSG was able to prevent the adhesion between leukocytes and endothelial cells, which supported
a plausible explanation for the antiseptic effects of QSSG.
QSSG, adhesion, adhesion molecules, antiseptic effect, endothelial cells, leukocytes.
Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.