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Current Molecular Imaging
ISSN (Print): 2211-5552
ISSN (Online): 2211-5544
VOLUME: 2
ISSUE: 2
DOI: 10.2174/22115552113029990001      Price:  $58









Fabrication of Split-Luciferase Complementation Assays for Molecular Imaging

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Author(s): Sung Bae Kim and Takeaki Ozawa
Pages 148-157 (10)
Abstract:
Recent revolutionary developments in luciferase engineering technologies now allow researchers to carry out quantitative imaging of molecular dynamics and cell signaling in living subjects, the technology of which includes protein-fragment complementation assay (PCA) and intein-mediated protein splicing assay (PSA). These technologies are functionally optimized with specific marine and beetle luciferases exerting designed properties and functionalities. This review overviews how to fabricate such bioluminescent tools and technologies of reconstituting split-luciferases, providing an excellent platform for today’s molecular imaging.
Keywords:
Bioluminescence, luciferase, protein-fragment complementation assay (PCA), single-chain probes.
Affiliation:
National Institute of Advanced Industrial Science and Technology (AIST), Japan.