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Current Drug Targets
ISSN (Print): 1389-4501
ISSN (Online): 1873-5592
Epub Abstract Ahead of Print
DOI: 10.2174/13894501113146660225      Price:  $95









Genomic identification of potential targets unique to Candida albicans for the discovery of antifungal agent

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Author(s): Himanshu Tripathi, Suaib Luqman, Abha Meena and Feroz Khan
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Abstract:
Despite of modern antifungal therapy, the mortality rates for invasive infection with human fungal pathogen Candida albicans is up to 40%. Studies suggest that drug resistance in the three most common species of human fungal pathogens viz., C. albicans, Aspergillus fumigatus (causes mortality rate up to 90%) and Cryptococcus neoformans (causes mortality rate up to 70%) are due to developing mutation in the targeted enzymes or through high expression of drug transporter genes. Drug resistance in human fungal pathogens has led to an imperative need for identification of new targets unique to fungal pathogens. In the present study, we have used a comparative genomics approach to find out potential target proteins unique to C. albicans, an opportunistic fungus responsible for severe infection in immuno compromised human. Interestingly many target proteins of existing antifungal agents showed orthologs in human cells. To identify unique proteins, we have compared proteome of C. albicans [SC5314] i.e., 14,633 total proteins retrieved from RefSeq database of NCBI, USA with proteome of human and non-pathogenic yeast Saccharomyces cerevisiae. Results showed that 4,568 proteins were identified unique to C. albicans in compared to human and later when these unique proteins compared with S. cerevisiae proteome, finally 2,161 proteins were identified as unique proteins and after removing repeats total 1,618 unique proteins (42 functionally known, 1,566 hypothetical and 10 unknown) are selected as potential antifungal drug targets unique to C. albicans
Affiliation:
Department of Metabolic and Structural Biology, CSIR-Central Institute of Medicinal & Aromatic Plants, Lucknow- 226015, India