The aim of the study was to determine the allele and genotype frequencies of rs1532624 SNP of the cholesteryl
ester transfer protein gene (CETP) among 116 Jordanian patients taking statins, and to study the impact of the genotypes
on response to statin therapy. The study was approved by the Institutional review Board (IRB) of The Jordan University
Hospital. An informed consent was signed by every participant. A single fragment encoding a 307 bp sequence of the
CETP gene, including the SNP of interest at position 14645 in intron 7, was amplified using a polymerase chain reaction
(PCR) technique directly from whole blood. The PCR product was then subjected to DNA sequencing. The frequencies of
the genotypes of the homozygous minor allele (AA), the homozygous major allele (CC), and the heterozygous allele (CA)
were 0.121, 0.405, and 0.474, respectively. The minor allele (A) frequency was 0.358. The frequencies did not deviate
from Hardy-Weinberg equilibrium. The lipid profile before the start of statin therapy was similar for all genotypes regarding
total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), and triglycerides, while high density lipoprotein
cholesterol (HDL-C) was significantly higher in the AA genotype. The AA genotype was also associated with significantly
lower CETP activity than the other genotypes. The response to statin therapy was less in the AA genotype than the
other genotypes for TC and LDL-C. In conclusion, the homozygous minor allele subjects have higher base line HDL-C,
and lower CETP activity than the other genotypes (CA and CC). They also have less reduction in TC and LDL-C after
CETP, CETP activity, jordanians, lipid profiles, rs1532624 polymorphism, statin users.
Department of Pharmacology, Faculty of Medicine, The University of Jordan, Amman 11942, Jordan.