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Recent Patents on Biotechnology
ISSN (Print): 1872-2083
ISSN (Online): 2212-4012
VOLUME: 7
ISSUE: 3
DOI: 10.2174/18722083113076660010      Price:  $100









Mini-Review: Recent Developments in Hydroxynitrile Lyases for Industrial Biotechnology

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Author(s): Elisa Lanfranchi, Kerstin Steiner, Anton Glieder, Ivan Hajnal, Roger A. Sheldon, Sander van Pelt and Margit Winkler
Pages 197-206 (10)
Abstract:
Hydroxynitrile lyases (HNLs) catalyze the cleavage as well as the formation of cyanohydrins. The latter reaction is valuable for the stereoselective C-C bond formation by condensation of HCN with carbonyl compounds. The resulting cyanohydrins serve as versatile building blocks for a broad range of chemical and enzymatic follow-up reactions. A significant number of (R)- and (S)-selective HNLs are known today and the number is still increasing. HNLs not only exhibit varying substrate scope but also differ in sequence and structure. Tailor-made enzymes for large-scale manufacturing of cyanohydrins with improved yield and enantiomeric excess are very interesting targets, which is reflected in a solid number of patents. This review will complement and extend our recent review with a strong focus on applications of HNLs for the synthesis of highly functionalized, chiral compounds with newest literature, recent and current patent literature.
Keywords:
Asymmetric synthesis, biocatalysis, biotransformation, cascade reactions, C-C coupling, chiral building blocks, cyanogenesis, cyanohydrin, enantioselectivity, enzyme engineering, HNL, hydrocyanic acid, hydroxynitrile lyase, industrial process, mandelonitrile lyase, plant enzymes, oxynitrilase, stereoselective.
Affiliation:
ACIB GmbH (Austrian Centre of Industrial Biotechnology GmbH), Petersgasse 14/III, A-8010 Graz, Austria.