Rooteomics: The Challenge of Discovering Defense-Related Proteins in Plants-An Update
Angela Mehta, Beatriz S. Magalhães, Djair S.L. Souza, Erico A.R. Vasconcelos, Luciano P. Silva, Maria F. Grossi-de-Sa, Octávio L. Franco and Thales L. Rocha
Chapter DOI: 10.2174/9781608054879113010018
In recent years, a strong emphasis has been given in deciphering the function
of genes unraveled by the completion of several genome sequencing projects. In plants,
functional genomics has been massively used in order to search for gene products of
agronomic relevance. As far as root-pathogen interactions are concerned, several genes
are recognized to provide tolerance/resistance against potential invaders. Root proteins
have also been increasingly identified, especially by using recent gel-free proteomic
techniques. However, root proteomic analysis is still a challenge, since the major
drawbacks, such as low proteins/volume content and high concentration of interfering
substances (pigments and phenolic compounds, among others) have not been overcome.
This scenario is further hindered by the overall low intrinsic amounts of root tissues in
many of the important target plants. The proteome analysis of plant-pathogen
interactions provides important information about the global proteins expressed in roots
in response to biotic stresses. Several pathogenic proteins superimpose the plant
proteome and can be identified and used as targets for the control of viruses, bacteria,
fungi and nematode pathogens. The present review focuses on advances in different
proteomic strategies dedicated to the challenging analysis of plant defense proteins
expressed during bacteria-, fungi- and nematode-root interactions. Recent
developments, limitations of the current techniques, and technological perspectives for
root proteomics aiming at the identification of resistance-related proteins are discussed.
Root, proteomics, fungi, bacteria, nematode, bidimensional
electrophoresis, mass spectrometry, protein identification, protein extraction,
plant-microorganism interaction, defense, gel-free, liquid chromatography.
Embrapa Recursos Genéticos e Biotecnologia, PqEB Av. W/5 Norte Final, Asa Norte, CEP 70770-900 Brasília, DF, Brazil.