Flooding stress restricts soybean growth, it results in decrease the production. In this report, to understand how
nuclear proteins in soybean affected by flooding, abundance changes of those proteins was analyzed. Nuclear proteins
were extracted from the root tips of soybean treated with or without flooding stress. The extracted proteins were analyzed
using a label-free quantitative proteomic technique. Of a total of 94 nuclear proteins that were found to be responsive to
flooding, the 19 and 75 proteins were increased and decreased, respectively. The identified flooding-responsive proteins
were functionally classified, revealing that 8 increased proteins changed in protein synthesis, posttranslational modification,
and protein degradation, while 34 decreased proteins were involved in transcription, RNA processing, DNA synthesis,
and chromatin structure maintenance. Among these proteins, those whose levels changed more than 10 fold included
two poly ADP-ribose polymerases and a novel G-domain-containing protein that might be involved in RNA binding. The
mRNA expression levels of these three proteins indicated a similar tendency to their protein abundance changes. These results
suggest that acceleration of protein poly-ADP-ribosylation and suppression of RNA metabolism may be involved in
root tip of soybean under flooding stress.
Flooding, nuclear, proteomics, soybean, root tip, protein poly-ADP-ribosylation, RNA metabolism.
National Institute of Crop Science, National Agriculture and Food Research Organization, 2-1-18 Kannondai, Tsukuba 305-8518, Japan