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Current Stem Cell Research & Therapy
ISSN (Print): 1574-888X
ISSN (Online): 2212-3946
VOLUME: 9
ISSUE: 2
DOI: 10.2174/1574888X08666131124135654      Price:  $58









Novel Chemically Modified Bacterial Cellulose Nanocomposite as Potential Biomaterial for Stem Cell Therapy Applications

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Author(s): Gerson Arisoly Xavier Acasigua, Gabriel Molina de Olyveira, Ligia Maria Manzine Costa, Daikelly Iglesias Braghirolli, Anna Christina Medeiros Fossati, Antonio Carlos Guastaldi, Patricia Pranke, Gildásio de Cerqueira Daltro and Pierre Basmaji
Pages 117-123 (7)
Abstract:
Bacterial cellulose (BC) has become established as a remarkably versatile biomaterial and can be used in a wide variety of applied scientific applications, especially for medical devices. In this work, the bacterial cellulose fermentation process is modified by the addition of hyaluronic acid and gelatin (1% w/w) to the culture medium before the bacteria is inoculated. Hyaluronic acid and gelatin influence in bacterial cellulose was analyzed using Transmission Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Adhesion and viability studies with human dental pulp stem cells using natural bacterial cellulose/hyaluronic acid as scaffolds for regenerative medicine are presented for the first time in this work. MTT viability assays show higher cell adhesion in bacterial cellulose/gelatin and bacterial cellulose/ hyaluronic acid scaffolds over time with differences due to fiber agglomeration in bacterial cellulose/gelatin. Confocal microscopy images showed that the cell were adhered and well distributed within the fibers in both types of scaffolds.
Keywords:
Bacterial cellulose, cell viability study, nanoskin®, natural nanocomposites, regenerative medicine, stem cells.
Affiliation:
Department of Physical Chemistry- UNESP/Araraquara-SP, 14800-900, Brazil.