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Recent Patents on Biotechnology
ISSN (Print): 1872-2083
ISSN (Online): 2212-4012
VOLUME: 7
ISSUE: 3
DOI: 10.2174/1872208307666131202192453      Price:  $100









Kinetic Properties and Role of Bacterial Chitin Deacetylase in the Bioconversion of Chitin to Chitosan

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Author(s): Ahmed ElMekawy, Hanaa M. Hegab, Ashraf El-Baz and Samuel M. Hudson
Pages 234-241 (8)
Abstract:
Chitin is an extremely insoluble material with very limited industrial use; however it can be deacetylated to soluble chitosan which has a wide range of applications. The enzymatic deacetylation of various chitin samples was investigated using the bacterial chitin deacetylase (CDA), which was partially purified from Alcaligenes sp. ATCC 55938 growth medium and the kinetic parameters of the enzyme were determined. Also, the efficiency of biocatalyst recycling by immobilization technique was examined. CDA activity reached its maximum (0.419 U/ml) after 18 h of bacterial cultivation. When glycol chitin was used as a substrate, the optimum pH of the enzyme was estimated to be 6 after checking a pH range between 3 and 9, while the optimum temperature was found to be 35°C. Addition of acetate (100 mM) in the assay mixture resulted in 50% loss of enzyme activity. The Km value of the enzyme is 1.6 × 10-4 µM and Vmax is 24.7 µM/min. The average activity of CDA was 0.38 U/ml for both of immobilized and freely suspended cells after 18 h of bacterial growth. Some related patents are also discussed here.
Keywords:
Chitin deacetylase, phylogenetics, chitin, chitosan, kinetics, biopolymers, immobilization.
Affiliation:
Institute of Advanced Technology & New Materials, City of Scientific Research & Technology Application Borg Elarab, Alexandria, Egypt