Abstract
Gold nanoparticles have suitable chemical surfaces, are neutral and have resistant to surface reduction. Due to these properties gold nanoparticles are frequently used in biological fields as biosensors as well as targeted drug and antibody delivery systems. The aim of this study was the biosynthesis of gold nanoparticles using Escherichia coli and the conjugation of these nanoparticles with streptomycin in order to evaluate its effect on obligate anaerobic bacteria. For this purpose, gold nanoparticles were biosynthesized using E. coli DH5α and confirmed using UV/Vis spectroscopy, SEM, TEM and DLS analysis. These nanoparticles were conjugated with streptomycin and its effect was evaluated against E. coli, Clostridium perferingens and Clostridium botulinum. As a result of this study 5-20 nm nanoparticles were synthesized from 5 mM HAuCl4. Distilled water was shown to be a suitable solvent and agitation facilitate the reaction. A range of ratios of antibiotics to nanoparticle and also different concentrations of antibiotics had little effect on the efficiency of conjugation. Conjugation of antibiotics with the nanoparticles resulted in a significant reduction in MIC and MBC of streptomycin against E. coli. Interestingly, this conjugate was able to overcome natural resistance of C. perferingens and C. botulinum to streptomycin. The process of biological synthesis is more ecologically friendly than traditional methods. The improvements in the efficacy of the antibiotics warrant further in vivo studies.
Keywords: Aminoglycoside, anaerobic bacteria, biosynthesis, gold, nanoparticle, streptomycin.
Graphical Abstract
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Books
Nanoscale Field Effect Transistors: Emerging Applications
Nanoelectronics Devices: Design, Materials, and Applications Part II
Nanoelectronics Devices: Design, Materials, and Applications (Part I)
Role of Nanotechnology in Cancer Therapy
Synthesis and Applications of Semiconductor Nanostructures
Pathways to Green Nanomaterials: Plants as Raw Materials, Reducing Agents and Hosts
Applications of Nanomaterials in Medical Procedures and Treatments
Synthesis of Nanomaterials
Nanobiotechnology: Principles and Applications
Bio-Inspired Nanotechnology
30