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Current Medicinal Chemistry
ISSN (Print): 0929-8673
ISSN (Online): 1875-533X
VOLUME: 21
ISSUE: 22
DOI: 10.2174/0929867321666131212154153      Price:  $58









Microarray Technologies for Intracellular Kinome Analysis

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Author(s): T. Yamamoto, T. Mori and Y. Katayama
Pages 2542-2552 (11)
Abstract:
Microarray-based kinomics, which measure the enzymatic activity or the presence of intracellular protein kinases, are now regarded as alternative tools to conventional mass spectrometry-based kinomics for examining intracellular kinomics. Here, we reviewed the principal advantages, recent progress, and remaining problems of representative microarray- based kinomics, including substrate peptide and protein microarrays, anti-protein kinase antibody microarrays, and reverse protein microarrays. Microarray-based kinomics are not as good at quantitative evaluation of kinomics as the conventional mass spectrometry-based kinomics. However, their simplicity and high throughput make the microarraybased kinomics unique tools, being especially suited for a practical analysis; monitoring drug effects on cellular kinomics as a tool for drug development, and for the diagnosis and prognosis of diseases based on kinomics.
Keywords:
Antibody, companion diagnosis, diagnosis, drug development, drug screening, intracellular signaling, microarray, molecular targeted drug, order-made medicine, peptide, personalized medicine, prognosis, protein, protein kinase.
Affiliation:
Department of Applied Chemistry, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819- 0395, Japan.