Abstract
An accurate, simple and sensitive method for determination of tiopronin (TP, N-(2-mercaptopropionyl)- glycine, Thiola) in human urine has been devised. Tiopronin is an important compound and is mainly used as a drug to prevent kidney stones. It works by removing the extra cystine from the body. Therefore, the reliable assay of tiopronin in biological matrices and pharmaceutical preparations is highly desirable. The proposed analytical procedure consists of reduction of tiopronin dimmer and its unsymmetrical disulfides with endogenous thiols, derivatization with 2-chloro-1- methylquinolinium tetrafluoroborate (CMQT) followed by acetonitrile stacking in capillary electrophoresis separation and ultraviolet-absorbance detection of TP-CMQT derivative at 350 nm. Baseline electrophoretic separation was achieved using standard fused-silica capillary (effective length 47.5 cm, 50 µm i.d.) and 0.25 mol L-1, Tris/HCl buffer adjusted to pH 2.0. The lower limit of quantitation for derivatized TP in urine was 5 µmol/L. The calibration curve showed linearity in the range 5-160 µmol L-1 urine with a regression coefficient corresponding to 0.9987 and the relative standard deviation of the points of the calibration curve being lower than 7%. The method can be used for a pharmacokinetic study with human subjects.
Keywords: Acetonitrile stacking, capillary electrophoresis, derivatization, tiopronin, ultraviolet detection, urine.
Current Analytical Chemistry
Title:Capillary Electrophoresis Determination of Tiopronin in Human Urine After Derivatization with 2-chloro-1-methylquinolinium Tetrafluoroborate
Volume: 10 Issue: 3
Author(s): Pawel Kubalczyk, Grazyna Chwatko and Edward Bald
Affiliation:
Keywords: Acetonitrile stacking, capillary electrophoresis, derivatization, tiopronin, ultraviolet detection, urine.
Abstract: An accurate, simple and sensitive method for determination of tiopronin (TP, N-(2-mercaptopropionyl)- glycine, Thiola) in human urine has been devised. Tiopronin is an important compound and is mainly used as a drug to prevent kidney stones. It works by removing the extra cystine from the body. Therefore, the reliable assay of tiopronin in biological matrices and pharmaceutical preparations is highly desirable. The proposed analytical procedure consists of reduction of tiopronin dimmer and its unsymmetrical disulfides with endogenous thiols, derivatization with 2-chloro-1- methylquinolinium tetrafluoroborate (CMQT) followed by acetonitrile stacking in capillary electrophoresis separation and ultraviolet-absorbance detection of TP-CMQT derivative at 350 nm. Baseline electrophoretic separation was achieved using standard fused-silica capillary (effective length 47.5 cm, 50 µm i.d.) and 0.25 mol L-1, Tris/HCl buffer adjusted to pH 2.0. The lower limit of quantitation for derivatized TP in urine was 5 µmol/L. The calibration curve showed linearity in the range 5-160 µmol L-1 urine with a regression coefficient corresponding to 0.9987 and the relative standard deviation of the points of the calibration curve being lower than 7%. The method can be used for a pharmacokinetic study with human subjects.
Export Options
About this article
Cite this article as:
Kubalczyk Pawel, Chwatko Grazyna and Bald Edward, Capillary Electrophoresis Determination of Tiopronin in Human Urine After Derivatization with 2-chloro-1-methylquinolinium Tetrafluoroborate, Current Analytical Chemistry 2014; 10 (3) . https://dx.doi.org/10.2174/1573411010999131219101552
DOI https://dx.doi.org/10.2174/1573411010999131219101552 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Management and Treatment of Dengue and Chikungunya - Natural Products to the Rescue
Combinatorial Chemistry & High Throughput Screening Synthesis of β-Benzylsulfanyl-β-trifluoromethyl-α-amino Acids Via Michael Addition in the Presence of Et3N/LiBr
Letters in Organic Chemistry Mimotopes of Viral Antigens and Biologically Important Molecules as Candidate Vaccines and Potential Immunotherapeutics
Combinatorial Chemistry & High Throughput Screening Cardiac and Muscular Involvement in Idiopathic Inflammatory Myopathies: Noninvasive Diagnostic Assessment and the Role of Cardiovascular and Skeletal Magnetic Resonance Imaging
Inflammation & Allergy - Drug Targets (Discontinued) Crohns Targeted Therapy: Myth or Real Goal?
Current Drug Discovery Technologies Therapeutic Targeting of Chemokines with Monoclonal Antibodies
Current Immunology Reviews (Discontinued) Folic Acid Conjugated Chitosan Nanoparticles for Tumor Targeting of Therapeutic and Imaging Agents
Pharmaceutical Nanotechnology Mannose Binding Lectin: A Potential Biomarker for Many Human Diseases
Current Medicinal Chemistry Comparison of <i>In Vitro</i> and <i>In Vivo</i> Effects of Infliximab on Cytokine Production in Proliferating CD4+ T Cells in Patients with Rheumatoid Arthritis
Clinical Anti-Inflammatory & Anti-Allergy Drugs (Discontinued) Pulmonary Manifestations of Rheumatoid Arthritis, Psoriatic Arthritis and Peripheral Spondyloarthritis: Prevalence, Diagnostic Approach and Treatment Options
Current Rheumatology Reviews The Janus Face of CD4+CD25+ Regulatory T Cells in Cancer and Autoimmunity
Current Medicinal Chemistry An Update on Drug Interactions with the Herbal Medicine Ginkgo biloba
Current Drug Metabolism The DREAM of Pain Relief
Current Rheumatology Reviews High-density Lipoprotein (HDL) Dysfunction and the Future of HDL
Current Vascular Pharmacology Lymphoproliferative Lesions in IgG4-Related Disease
Current Immunology Reviews (Discontinued) Phospholipase Cγ Signaling in Bone Marrow Stem Cell and Relevant Natural Compounds Therapy
Current Stem Cell Research & Therapy Regulation of Ion Channels, Cellular Carriers and Na(+)/K(+)/ATPase by Janus Kinase 3
Current Medicinal Chemistry The Significance of Oxidative Stress in Articular Cartilage Ageing and Degradation
Current Rheumatology Reviews Anti-inflammatory Action of Green Tea
Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry Design, Synthesis and Characterization of N', N'-Diaryl Ureas as p38 Kinase Inhibitors
Medicinal Chemistry