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Current Catalysis
ISSN (Print): 2211-5447
ISSN (Online): 2211-5455
VOLUME: 3
ISSUE: 2
DOI: 10.2174/2211544702666131224234223      Price:  $58









Purification of The Lipase B of Candida antarctica from a Commercial Enzymatic Extract

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Author(s): Carlos R. Llerena-Suster, Laura E. Briand and Susana R. Morcelle
Pages 155-160 (6)
Abstract:
This paper presents a rational strategy to purify the lipase B of Candida antarctica of the commercial extract Lipozyme® through size exclusion coupled with anionic exchange chromatography using a non conventional, easy to remove buffer system such as ammonia-ammonium. In this context, each step of the purification was followed through the determination of the protein content, esterase activity measurements, SDS-PAGE, agarose electrophoresis, UVspectroscopy and isoelectric focusing. The purification of the commercial extract afforded a sample that retains 47% of the proteins (being CALB the major enzymatic component of the purified sample) with a hydrolytic activity higher than the starting crude extract.
Graphical Abstract:
Keywords:
Protein purification, CALB, UV absorbance, Bradford, size exclusion chromatography, Ion exchange chromatography.
Affiliation:
Centro de Investigación y Desarrollo en Ciencias Aplicadas-Dr. Jorge J. Ronco. Universidad Nacional de La Plata, CONICET, CCT La Plata. Calle 47 No 257, B1900AJK La Plata, Buenos Aires, Argentina.