In clinical islet transplantation, islet purification reduces the amount of tissues to be transplanted by removing
the acinar tissue, therefore minimizing the risks associated with intraportal infusion. On the other hand, the purification
procedure may result in decreased numbers of islets recovered from digested tissue and may be traumatic for the islets. Ficoll-
based density gradients are widely used in islet purification in clinical trials. Recently, purification with iodixanol was
reported in an islet transplantation series with successful clinical outcomes. This review describes current advances in islet
purification for clinical islet transplantation.
Density, islet isolation, islet transplantation, osmolality, regular purification, supplemental purification.
Department of Surgery, Chiba-East National Hospital, National Hospital Organization, 673 Nitonacho, Chuo-ku, Chiba 260-8712, Japan.