Influence of Physical Immobilization of dsDNA on Carbon Based Matrices of Electrochemical Sensors

Title:Influence of Physical Immobilization of dsDNA on Carbon Based Matrices of Electrochemical Sensors

Volume: 10 Issue: 1

Author(s): Livia Alexandra Gugoasa, Raluca-Ioana Stefan-van Staden, Anton Alexandru Ciucu and Jacobus Frederick van Staden

Affiliation:

Keywords: dsDNA, sensors, carbon based matrices, neurotransmitters.

Abstract: The influence of addition of dsDNA on the electrocatalytic effect of carbon matrices, e.g. synthetic monocrystalline diamond, fullerenes C₆₀ and multi-walled carbon nanotubes was studied. Three neurotransmitters: dopamine, epinephrine and norepinephrine, which are known as biomarkers that play an important role in transporting and modulating nervous impulses in the synapse were chosen as model analytes. Cyclic voltammetry was used to determine the optimum working conditions with a phosphate buffer solution at a pH=4 with KNO₃ as support electrolyte. Differential pulse voltammograms (DPVs) were employed to assess the electrolytic effect of dsDNA on carbon matrices under the optimized conditions, and the potential applications of the biosensors for pharmaceutical and biomedical analysis.

Cite this article as:

Gugoasa Alexandra Livia, Staden Stefan-van Raluca-Ioana, Ciucu Alexandru Anton and Staden Frederick van Jacobus, Influence of Physical Immobilization of dsDNA on Carbon Based Matrices of Electrochemical Sensors, Current Pharmaceutical Analysis 2014; 10 (1) . https://dx.doi.org/10.2174/157341291001140102104740