Abstract
Bone marrow stromal cells (BMSCs) were considered as one of the strongest candidates for cell transplantations to treat neurological disorders. Previously, we had showed that BMSCs isolated from rats could be induced to differentiate into neural cells being cocultured with olfactory ensheathing cells (OECs). In this study, we further demonstrated the neural differentiation of human BMSCs (hBMSCs) when cocultured with OECs and daily supplement of bFGF (basic fibroblast growth factor). Transwell culture dishes with a 0.4-mm pore size were used to coculture hBMSCs and OECs. At different time points (12h, 24h, 3d, 7d, 14d), the induced hBMSCs were morphologically observed and performed immunocytofluorescence and quantitative RT-PCR (qRT-PCR). The number of neural markers-positive cells significantly increased after coculture, and gene expression of NSE, β-III-tubulin, MAP2, GFAP also dramatically increased. Our study suggested that hBMSCs could be induced into neuron-like cells under conditions of coculture with OECs and daily supplement of bFGF. The differentiated autologous hBMSCs had a great potential for transplantation to treat CNS lesion.
Keywords: Basic fibroblast growth factor, coculture, human bone marrow stromal cells, neural cells, olfactory ensheathing cells.
Current Stem Cell Research & Therapy
Title:Induced Human Bone Marrow Stromal Cells Differentiate into Neural Cells by bFGF and Cocultured with Olfactory Ensheathing Cells
Volume: 9 Issue: 4
Author(s): Wen-Fei Ni, Ai-Min Wu, Qing-Long Li, Zhe-Yu Huang, Hua-Zi Xu and Li-Hui Yin
Affiliation:
Keywords: Basic fibroblast growth factor, coculture, human bone marrow stromal cells, neural cells, olfactory ensheathing cells.
Abstract: Bone marrow stromal cells (BMSCs) were considered as one of the strongest candidates for cell transplantations to treat neurological disorders. Previously, we had showed that BMSCs isolated from rats could be induced to differentiate into neural cells being cocultured with olfactory ensheathing cells (OECs). In this study, we further demonstrated the neural differentiation of human BMSCs (hBMSCs) when cocultured with OECs and daily supplement of bFGF (basic fibroblast growth factor). Transwell culture dishes with a 0.4-mm pore size were used to coculture hBMSCs and OECs. At different time points (12h, 24h, 3d, 7d, 14d), the induced hBMSCs were morphologically observed and performed immunocytofluorescence and quantitative RT-PCR (qRT-PCR). The number of neural markers-positive cells significantly increased after coculture, and gene expression of NSE, β-III-tubulin, MAP2, GFAP also dramatically increased. Our study suggested that hBMSCs could be induced into neuron-like cells under conditions of coculture with OECs and daily supplement of bFGF. The differentiated autologous hBMSCs had a great potential for transplantation to treat CNS lesion.
Export Options
About this article
Cite this article as:
Ni Wen-Fei, Wu Ai-Min, Li Qing-Long, Huang Zhe-Yu, Xu Hua-Zi and Yin Li-Hui, Induced Human Bone Marrow Stromal Cells Differentiate into Neural Cells by bFGF and Cocultured with Olfactory Ensheathing Cells, Current Stem Cell Research & Therapy 2014; 9 (4) . https://dx.doi.org/10.2174/1574888X09666140115114350
DOI https://dx.doi.org/10.2174/1574888X09666140115114350 |
Print ISSN 1574-888X |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-3946 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Co-Existence of GABA and Glu Transporters in the Central Nervous System
Current Topics in Medicinal Chemistry Emerging Evidence for Neurotensin Receptor 1 Antagonists as Novel Pharmaceutics in Neurodegenerative Disorders
Mini-Reviews in Medicinal Chemistry Drug Targets from Genetics: Alpha-Synuclein
CNS & Neurological Disorders - Drug Targets Imidazole: An Essential Edifice for the Identification of New Lead Compounds and Drug Development
Mini-Reviews in Medicinal Chemistry Mechanisms of Acupuncture Effect on Alzheimer’s Disease in Animal- Based Researches
Current Topics in Medicinal Chemistry Insights into the Role of Matrix Metalloproteinases and Tissue Inhibitor of Metalloproteinases in Health and Disease
Current Chemical Biology Cannabinoid Drugs and Enhancement of Endocannabinoid Responses: Strategies for a Wide Array of Disease States
Current Molecular Medicine Nitric Oxide, Peroxynitrite, Peroxynitrous Acid, Nitroxyl, Nitrogen Dioxide, Nitrous Oxide: Biochemical Mechanisms and Bioaction
Current Bioactive Compounds New Targets for Therapy in Polyglutamine (polyQ) Expansion Diseases
Current Drug Therapy Past, Present and Future of Nutrigenomics and its Influence on Drug Development
Current Drug Discovery Technologies Heat Shock Proteins Protect Against Ischemia and Inflammation Through Multiple Mechanisms
Inflammation & Allergy - Drug Targets (Discontinued) Warming Up to New Possibilities with the Capsaicin Receptor TRPV1: mTOR, AMPK, and Erythropoietin
Current Neurovascular Research Editorial (Thematic Issue: Cell Metabolism and Current Challenges in Drug Research)
Current Pharmaceutical Design Yeast as a Powerful Model System for the Study of Apoptosis Regulation by Protein Kinase C Isoforms
Current Pharmaceutical Design Analysis of NCL Proteins from an Evolutionary Standpoint
Current Genomics Editorial - Progress and Vision for ‘Current Aging Science’
Current Aging Science Cannabinoids as Therapeutic Agents for Ablating Neuroinflammatory Disease
Endocrine, Metabolic & Immune Disorders - Drug Targets Inhibitory Mechanism of An Anticancer Drug, Bexarotene Against Amyloid β Peptide Aggregation: Repurposing Via Neuroinformatics Approach
Current Pharmaceutical Design Management of Glia-Mediated Neuroinflammation and Related Patents
Recent Patents on Inflammation & Allergy Drug Discovery Biochemical Markers in CSF of ALS Patients
Current Medicinal Chemistry