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Current Drug Discovery Technologies
ISSN (Print): 1570-1638
ISSN (Online): 1875-6220
VOLUME: 11
ISSUE: 1
DOI: 10.2174/1570163811666140121154649









Different Approaches for Obtaining Antibodies from Human B Cells

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Author(s): Marcus R. Duvall and Ryan N. Fiorini
Pages 41-47 (7)
Abstract:
Antibodies have emerged as powerful therapeutics effective for treating a number of human conditions and diseases. While early successes utilized small animals to generate therapeutic antibodies, human antibodies are now preferred in order to limit anti-antibody immune responses. Antibodies with human amino acid sequences can be generated in a number of ways, such as humanizing antibodies from other species or expressing human antibodies in transgenic animals. This review focuses on methods for obtaining antibodies directly from human B cells. These methods use both antigen exposed and non-exposed (“naïve”) humans as B cell sources, and apply various technologies to isolate desired antibodies; including cell line generation, single cell isolation, display technologies, and B cell library generation.
Keywords:
Antibody engineering, antibody libraries, display technologies, high-throughput screening, human monoclonal antibodies, human B cell.
Affiliation:
13 Lowndes Pointe Drive, Charleston, 29403, USA.