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Current Stem Cell Research & Therapy
ISSN (Print): 1574-888X
ISSN (Online): 2212-3946
DOI: 10.2174/1574888X09666140213150218

Recent Advances in Cell Sheet Technology for Periodontal Regeneration

Author(s): Jing Wang, Rui Zhang, Yun Shen, Chenyuan Xu, Shengcai Qi, Liyan Lu, Raorao Wang and Yuanzhi Xu
Pages 162-173 (12)
Tissue engineering has yielded several successes in early clinical trials of regenerative medicine with grafting therapeutic cells seeded into biodegradable scaffolds. However this conventional cell delivery method has limited the field’s progress. In recent decades, we have developed a novel cell transferring method, cell sheet technology that allows for controlled attachment and detachment of cells via simple temperature variations of a surface-intelligent temperatureresponsive polymer:poly (N-isopropylacrylamide). It has been widely applied to create functional tissue sheets with cells derived from various tissues to treat a wide range of diseases. Periodontal cell sheets non-invasively harvested from temperature- responsive culture surfaces have been successfully manufactured, resulting in communicative multilayered constructs. Transplantation of cell sheets onto periodontal defects has improved bone and tissue regeneration in animal models and humans and shows low immunogenicity. In this review, we summarize the recent advances of techniques in cell sheet engineering and its application for periodontal regeneration.
Application, cell sheet technology, co-culture, micro-printing, periodontal regeneration, tissue engineering.
Department of Stomatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, No. 301, Middle Yanchang Road, Shanghai 200072, P.R. China.