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Drug Delivery Letters
ISSN (Print): 2210-3031
ISSN (Online): 2210-304X
DOI: 10.2174/2210303104666140222002510      Price:  $58

TiO2~Deoxyribozyme Nanocomposites as Delivery System and Efficient Site-Specific Agents for Cleavage of RNA Targets

Author(s): Asya S. Levina, Marina N. Repkova, Nadezhda V. Shikina, Zinfer R. Ismagilov, Natalia V. Shatskaya and Valentina F. Zarytova
Pages 142-147 (6)
Synthetic DNA molecules of 10-23 deoxyribozymes (Dz) are known to be efficient and site-specific agents for the RNA cleavage in a catalytic manner. To provide the penetration of Dz into cells, we have proposed the new delivery system for deoxyribozymes in the form of TiO2•PL-Dz nanocomposite. These nanocomposites consist of catalytically active Dz molecules noncovalently immobilized through the polylysine linker to TiO2 nanoparticles, which provide the penetration of Dz into cells. Deoxyribozymes in the prepared TiO2•PL-Dz nanocomposites were shown to retain their ability to cleave RNA targets albeit at a slower rate but with the same site-specificity and similar efficiency as free Dz. Unlike free Dz, the proposed TiO2•PL-Dz nanocomposites penetrate into cells without auxiliary actions.
Delivery, deoxyribozymes, immobilization, nanocomposites, TiO2 nanoparticles.
Institute of Chemical Biology and Fundamental Medicine, Siberian branch of RAS, pr. Lavrent’eva 8, Novosibirsk, 630090, Russia.