Isolation and Identification of a Phenol-Degrading Yeast JP-1 with High Activity and Its Degrading Characteristics and Kinetic
Fei Pan, Ai-Hua Xu, Qing-Fu Zeng, Xin Zhao and Hai-Liang Zhu
Pages 4-9 (6)
A yeast strain with high activity of phenol degradation was isolated from activated sludge of a wastewater
treatment plant in a coking chemical factory, and identified as Candida tropicalis according to the analysis of VITEK-32
and its 18SrDNA gene sequence. The strain designated as JP-1 was able to grow on phenol as a sole carbon and energy
source and it can degrade more than 90% of 1800 mg⋅L-1 phenol within 72h. The optimum pH was 6.0. Kinetic equation
of phenol degradation was obtained by regression using MATLAB software. The parameters of Haldane equation were
following: µmax=0.387 l⋅hr-1, Ks=4.20 mg⋅L-1, KiKi =465.6 mg⋅L-1. Cell grew was obviously inhibition by high
concentration phenol and the inhibition strengthen increased with phenol increasing.
Biodegradation, Candida tropicalis, kinetic, Haldane equation, phenol, wastewater treatment.
School of Life Science, Nanjing University, Nanjing 210093, China.