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Combinatorial Chemistry & High Throughput Screening
ISSN (Print): 1386-2073
ISSN (Online): 1875-5402
VOLUME: 17
ISSUE: 4
DOI: 10.2174/1386207317666140323133249









Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute

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Author(s): Emma J. Shanks
Pages 328-332 (5)
Abstract:
The RNAi Screening Facility at the Cancer Research UK Beatson Institute combines siRNA genome-wide screening with drug screening coupled with High Content Imaging and fluorescence-based phenotypic assays to target multiple types of cancer. Here, we describe the infrastructure of the Facility and the approaches we utilise. We also share our experiences in running such a facility and developing and executing screening campaigns, with particular regard to high content multiparametric analysis, data management and statistical analysis.
Keywords:
Cancer, high content analysis, phenotypic screening, RNAi screening, statistical analysis.
Affiliation:
Cancer Research UK Beatson Institute, Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK.