Approach: The analgesic activity was determined for its central and peripheral pharmacological actions using Eddy’s hot plate method and acetic acid-induced writhing test in mice respectively. The ethanol, methanol and aqueous extracts were also investigated for its antioxidant potential through 1, 1- diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and Nitric Oxide (NO) scavenging assays.
Results: Methanol and aqueous extract at the doses 200, 400 mg/kg and ethanol extract at high dose (400 mg/kg) significantly (p<0.05) inhibited number of writhing as well as increase in mean latency time. The ethanol extract at low dose (200 mg/kg) showed centrally anti-nociceptive effect but not peripherally. In acetic acid-induced writhing test, the ethanol, aqueous and methanol extract at the dose of 400 mg/kg produced a maximum of 81.21%, 79.18% and 72.5 % inhibition whereas reference drug Indomethacin showed a maximum inhibition of 79.26 % respectively. A dose dependent scavenging of DPPH radical scavenging has been observed with IC50 values of the methanol, ethanol and aqueous extracts were found to be 65.50, 167.4 & 85.85 µg/ml whereas the IC50 value for the reference ascorbic acid was 52.34 µg/ml. Extracts were also found to possess good nitric oxide scavenging potential with IC50 value of 220.43, 150.45 and 310.25 µg/ml of ethanol, methanol and aqueous extracts while IC50 value of standard ascorbic acid was found to be the 125.10 µg/ml respectively.