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Infectious Disorders - Drug Targets
(Formerly Current Drug Targets - Infectious Disorders)
ISSN (Print): 1871-5265
ISSN (Online): 2212-3989
VOLUME: 13
ISSUE: 5
DOI: 10.2174/187152651305140403122334      Price:  $58









Identification of L-Amino Acid Oxidase (Mb-LAAO) with Antibacterial Activity in the Venom of Montivipera bornmuelleri, a Viper from Lebanon

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Author(s): Mohamad Rima, Claudine Accary, Katia Haddad, Riyad Sadek, Souad Hraoui-Bloquet, Jean C. Desfontis and Ziad Fajloun
Pages 337-343 (7)
Abstract:
The L-amino acid oxidase (LAAO) is a multifunctional enzyme, able to partake in different activities including antibacterial activity. In this study, a novel LAAO (Mb-LAAO) was isolated from the venom of M. bornmuelleri snake using size exclusion chromatography followed by RP-HPLC and partially characterized. However, the molecular weight of the Mb-LAAO determined by ESI-MS and SDS-PAGE was 59 960.4 Da. Once the enzymatic activity test confirming the enzyme’s identity (transformation of L-leucine) was done, the Mb-LAAO was evaluated for its antibacterial activity against Gram-negative bacteria. It showed a remarkable effect against M. morganii and K. pneumoniae. Moreover, no cytotoxic activity was observed for Mb-LAAO against human erythrocytes arguing for an exploration of its pharmaceutical interest.
Keywords:
Antibacterial effect, LAAO activity, L-amino acid oxidase, LC-ESI-MS analysis, Montivipera bornmuelleri.
Affiliation:
Azm Center for Research in Biotechnology and its Applications, Doctoral School of Science and Technology, Lebanese University, El Mittein Street, Tripoli, Lebanon.