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Protein & Peptide Letters
ISSN (Print): 0929-8665
ISSN (Online): 1875-5305
VOLUME: 21
ISSUE: 9
DOI: 10.2174/0929866521666140411113021      Price:  $58









Separation and Purification of Small Peptides from Fermented Sesame Meal and Their Antioxidant Activities

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Author(s): Xu Fazhi, Peng Huihui, Li Yang, Li Lumu, Qian Kun and Ding Xioling
Pages 966-974 (9)
Abstract:
Protein hydrolysates are widely applied as antioxidants in nutrition, but the potential antioxidant activities of small peptides remain unknown. Therefore, we investigated the antioxidant activities of small peptides isolated from solid-state fermented sesame meal via Sephadex G-15 chromotography. The scavenging capacities for 2, 2- diphenylpicrylhydrazyl (DPPH) and hydroxyl (•OH) radicals as well as the total reducing capacity were determined. The in vivo antioxidant activity was determined upon 30-d intragastric administration of the isolated small peptides (tripeptide, tetrapeptide, and hexapeptide) at different doses (0.1, 0.2, and 0.4 g/kg•d) in healthy Kunming mice. The results showed that the DPPH and •OH scavenging rates of the three peptides exceeded 80%. The total reducing activities of 4 mg/mL tetrapeptide or hexapeptide and 2 mg/mL tripeptide were comparable to that of 0.5 mg/mL glutathione. In mice fed sesame peptides, malondialdehyde levels in the serum and liver were lower than those in controls, whereas the activities of liver superoxide dismutase and glutathione peroxidase were significantly higher than those in controls (P<0.05). The antioxidant activity of tripeptide was significantly higher than those of tetrapeptide and hexapeptide (P<0.05). In conclusion, small peptides extracted from solid-state fermented sesame meal possess strong antioxidant activities that increase with decreasing peptide molecular weight.
Graphical Abstract:
Keywords:
Antioxidant activity, fermented sesame meal, separation and purification, small peptides.
Affiliation:
School of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.