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Current Drug Delivery
ISSN (Print): 1567-2018
ISSN (Online): 1875-5704
Epub Abstract Ahead of Print
DOI: 10.2174/1567201811666140515101130      Price:  $95









Influence of Gold Nanoparticle Tagged Snake Venom Protein Toxin NKCT1 on Ehrlich Ascites Carcinoma (EAC) and EAC Induced Solid Tumor Bearing Male Albino Mice

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Author(s): Tanmoy Bhowmik, Partha Pratim Saha, Anjan Kumar DasGupta and Antony Gomes
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Abstract:
It was earlier reported that gold nanoparticle (GNP) and snake venom protein toxin NKCT1 was conjugated and primary characterization was done. In the present communication, further characteristics of GNP-NKCT1 were done with SEM, EDS, XRD and Raman spectra for its physio-chemical nature and bonding. SEM showed the formation of goldnanoparticles, whereas EDS and XRD confirmed the 60-90% goldnanoparticle in the solution. Raman shift corresponding to (C=O), (N-H), (C-N) confirmed the proper conjugation of GNP with NKCT1. GNP-NKCT1 showed anticancer effect both in vivo and in vitro in EAC cell and antitumor effect in EAC induced mice. In in vivo studies GNP-NKCT1 increased MST 108.30% and decreased 51.39% viable EAC cell count. Fluorescent micrograph showed signs of apoptosis (membrane blebbing, membrane disruption). Decrease level of IL-10 and low incorporation of BrdU showed decreased proliferation of EAC induced by GNP-NKCT1. With upregulation of Bax, down regulation of Bcl2 and increase expression of caspase 3/9, it was confirmed that GNP-NKCT1 induced caspase dependent apoptosis pathway in EAC cell. In in vitro studies, GNP-NKCT1 increased the late apoptotic stage of cell and arrested cell cycle division at G0/G1 state. GNP-NKCT1 also decreased tumor volume and tumor weight in EAC induced tumor in male albino mice. It inhibited the angiogenesis, which was confirmed by lower percentage of expression of VEGF. This study indicated the capability of gold nanoparticles which enhanced the tumor uptake of NKCT1 and also suggested that GNP-NKCT1 might be a good source for anti-carcinoma and anti-tumor agent
Affiliation:
Laboratory of Toxinology & Experimental Pharmacodynamics Department of Physiology, University of Calcutta 92, APC Road, Kolkata -700 009, India