In-vitro Studies on α-Amylase, α-Glucosidase and Aldose Reductase Inhibitors found in Endophytic Fungi Isolated from Ocimum sanctum

Title:In-vitro Studies on α-Amylase, α-Glucosidase and Aldose Reductase Inhibitors found in Endophytic Fungi Isolated from Ocimum sanctum

Volume: 10 Issue: 2

Author(s): Pavithra N., Sathish L., Suneel Kumar A., Venkatarathanamma V., Pushpalatha H., Bhanuprakash Reddy G. and Ananda Kulal

Affiliation:

Keywords: Anti-diabetic, endophytic fungi, enzyme inhibitors, medicinal plant, molecular sequence.

Abstract: Eighty four endophytic fungi were isolated from Ocimum sanctum. Secondary metabolites extracted from these endophytic fungi were tested for the inhibitors of enzymes responsible for the glycaemic index and polyol pathway. Six isolates were positive for both α-glucosidase and α-amylase inhibitors. Secondary metabolites from four endophytic fungal isolates inhibited aldose reductase enzyme extracted from rat lens. Crude extracts of fungal isolates POST075 and POST083 were found to be the most potent α-glucosidase inhibitors among the active isolates with IC₅₀ values of 29.51 and 31.26 μg/mL respectively. Whereas, ethyl acetate extracts of fungal isolates POST034 and POST075 showed highest inhibition for pancreatic α-amylase enzyme in-vtiro with an IC₅₀ value of 27.34 and 40.73 μg/mL respectively. The IC₅₀ value for α-amylase enzyme inhibitors of POST034 and POST075 was very close to IC₅₀ value of standard drug acarbose (25.7 μg/mL) tested under similar conditions. Crude extract of endophytic fungal isolate POST047 was found to be an effective aldose reductase inhibitor with an IC₅₀ value of 29 μg/mL when compared to the IC₅₀ value of standard drug quercetin that has an IC₅₀ value of 15 μg/mL under similar condition. Fungal isolates with best inhibition activity were identified as Alternaria tenuissima (POST34), Trichoderma sp. (POST047), Diaporthe sp (POST075) and Colletotrichum gloesporioides (POST083) by the rDNA molecular method.

Cite this article as:

N. Pavithra, L. Sathish, A. Kumar Suneel, V. Venkatarathanamma, H. Pushpalatha, G. Reddy Bhanuprakash and Kulal Ananda, In-vitro Studies on α-Amylase, α-Glucosidase and Aldose Reductase Inhibitors found in Endophytic Fungi Isolated from Ocimum sanctum, Current Enzyme Inhibition 2014; 10 (2) . https://dx.doi.org/10.2174/1573408011666141205233252