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Recent Patents on Signal Processing (Discontinued)

Editor-in-Chief

ISSN (Print): 2210-6863
ISSN (Online): 1877-6124

Effects of Photon Losses on Fluorescence Lifetime Imaging Microscopy (FLIM) System Optimization

Author(s): Lior Turgeman, Tsviya Nayhoz, Nir Roth, Gilad Yahav, Avraham Hirshberg and Dror Fixler

Volume 4, Issue 2, 2014

Page: [67 - 77] Pages: 11

DOI: 10.2174/2210686305666150128201251

Price: $65

Abstract

Several approaches for optimization of fluorescence lifetime imaging microscopy (FLIM) system have been recently suggested. This paper discusses the influences of photons losses on the optimization of FLIM systems based time-correlated single photon counting (TCSPC) technique, considering the limitations associated with detecting the required amount of photons by the system. The fluorescence intensity (FI) and fluorescence lifetime (FLT) were measured in different operating regimes of the imaging system. The relation between parameters such as excitation power, detector gain, laser repetition rate, is also analyzed. Based on data acquisition limitations of typical TCSPC systems, we discuss the considerations for choosing the correct system parameters, which would most influence the accuracy of FLIM experiments. A simple scheme for patent optimization of FLIM systems for different types of fluorescent samples is finally suggested.

Keywords: Fluorescence lifetime imaging, intra-pulse pile-up effect, photon losses, signal to noise ratio, system optimization, time correlated single photon counting.

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