Abstract
In this research, greener chromatography employing a more eco-friendly microemulsion mobile phase was utilized for the analysis of rosiglitazone (ROS) and glimepiride (GLM) in rat sera. ROS, GLM, and darfenacin (an internal standard) were detected using UV wavelength of 237 nm. HPLC analysis by using a monolithic column was performed by directly injecting the sample after appropriate dilution with the mobile phase. The chromatographic behavior of these compounds was studied to demonstrate their chromatographic efficiency, retention, and peak symmetry. Regression analyses showed excellent linearity (r = 0.999) over concentrations ranging from 100 to 5000 ng mL-1for both ROS and GLM. The developed method by using rat sera had limits of detection of 40.0 ng mL-1for both drugs. The assay was statistically validated for linearity, accuracy, precision and specificity according to ICH guidelines. The intra- and inter-assay precisions were satisfactory and the accuracy of the method was proved. This assay was successfully applied in a pharmacokinetic study.
Keywords: Green chromatography, monolithic column, rosiglitazone and glimepiride, rat sera, pharmacokinetics.
Graphical Abstract
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
Bioanalytical and Biosensor Technologies for Target Molecules
Bioanalytical and biosensor technologies constitute the core of detecting and sensing diverse molecules within living organisms, encompassing proteins, DNA, small molecular metabolites, and beyond. These molecules assume pivotal roles in biological processes, rendering their analysis and sensing indispensable for comprehending biological phenomena and unraveling disease mechanisms. In the current landscape ...read more
Related Journals
Anti-Cancer Agents in Medicinal Chemistry
Current Computer-Aided Drug Design
Current Bioactive Compounds
Combinatorial Chemistry & High Throughput Screening
Current Medicinal Chemistry
The Natural Products Journal
Current Catalysis
Current Chinese Chemistry
Current Chinese Science
Current Topics in Chemistry
Related Books
Botanicals and Natural Bioactives: Prevention and Treatment of Diseases
Biosurfactants: A Boon to Healthcare, Agriculture & Environmental Sustainability
Frontiers in Computational Chemistry
Advances in Dye Degradation
Biological and Medical Significance of Chemical Elements
Frontiers In Medicinal Chemistry
Advances in Organic Synthesis
Frontiers in Natural Product Chemistry
Recent Advances in Analytical Techniques
Advanced Catalysts Based on Metal-organic Frameworks (Part 2)
31
1
1
1