Abstract
Cassava (Manihot esculenta Crantz) belongs to the Euphorbiaceae family and is originated from the Southern Amazon basin. The storage root is the most important product of cassava as food for more than 800 million people in Africa, Asia and Latin America. In this review, we present a retrospective of studies aiming the identification of cassava proteins, starting from the first investigations using SDS-PAGE and classical two-dimensional gel electrophoresis (2DE) to recent studies with advanced technologies such as high-resolution 2DE, mass spectrometry, and iTRAQ-based analysis that have contributed for characterization of cassava proteome. Several cassava proteins have been identified, including those involved in the storage root formation and post-harvest physiological deterioration processes.
Keywords: Cassava, mass spectrometry, post-harvest physiological deterioration, proteomics, storage root formation, uni- and bi-dimensional gel electrophoresis.
Current Protein & Peptide Science
Title:An Overview of Protein Identification Studies in Cassava
Volume: 16 Issue: 3
Author(s): Claudia R. Batista de Souza, Savio P. dos Reis and Luiz J. Castelo Branco Carvalho
Affiliation:
Keywords: Cassava, mass spectrometry, post-harvest physiological deterioration, proteomics, storage root formation, uni- and bi-dimensional gel electrophoresis.
Abstract: Cassava (Manihot esculenta Crantz) belongs to the Euphorbiaceae family and is originated from the Southern Amazon basin. The storage root is the most important product of cassava as food for more than 800 million people in Africa, Asia and Latin America. In this review, we present a retrospective of studies aiming the identification of cassava proteins, starting from the first investigations using SDS-PAGE and classical two-dimensional gel electrophoresis (2DE) to recent studies with advanced technologies such as high-resolution 2DE, mass spectrometry, and iTRAQ-based analysis that have contributed for characterization of cassava proteome. Several cassava proteins have been identified, including those involved in the storage root formation and post-harvest physiological deterioration processes.
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Cite this article as:
Batista de Souza Claudia R., P. dos Reis Savio and Branco Carvalho Luiz J. Castelo, An Overview of Protein Identification Studies in Cassava, Current Protein & Peptide Science 2015; 16 (3) . https://dx.doi.org/10.2174/1389203716666150224145407
DOI https://dx.doi.org/10.2174/1389203716666150224145407 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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