Abstract
Herein, we describe a novel, microwave-assisted synthesis of a binding-based probe capable of enriching proteins that bind adenine ribose derivatives (AdoRs). We employed this probe in activity- based protein profiling studies to qualitatively assess the AdoR-binding proteome in three bacterial cell lines from the genus Bacillus. This proof of concept experiment demonstrated a unique subset of proteins distinctive to each species, and confirmed the efficacy of the probe tagging and subsequent enrichment.
Keywords: AdoRs, binding-based probe (BBP) binding-based protein profiling (BBPP), microwave-assisted synthesis.
Current Microwave Chemistry
Title:A Facile, Microwave-Assisted Synthesis of an Adenosine-Ribose Probe for Binding-Based Profiling of Nucleoside and Nucleotide-Binding Proteins
Volume: 3 Issue: 2
Author(s): Matthew R. Battistini, Shikha Mahajan, Daili Diaz, Lindsey N. Shaw and David J. Merkler
Affiliation:
Keywords: AdoRs, binding-based probe (BBP) binding-based protein profiling (BBPP), microwave-assisted synthesis.
Abstract: Herein, we describe a novel, microwave-assisted synthesis of a binding-based probe capable of enriching proteins that bind adenine ribose derivatives (AdoRs). We employed this probe in activity- based protein profiling studies to qualitatively assess the AdoR-binding proteome in three bacterial cell lines from the genus Bacillus. This proof of concept experiment demonstrated a unique subset of proteins distinctive to each species, and confirmed the efficacy of the probe tagging and subsequent enrichment.
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Cite this article as:
Battistini R. Matthew, Mahajan Shikha, Diaz Daili, Shaw N. Lindsey and Merkler J. David, A Facile, Microwave-Assisted Synthesis of an Adenosine-Ribose Probe for Binding-Based Profiling of Nucleoside and Nucleotide-Binding Proteins, Current Microwave Chemistry 2016; 3 (2) . https://dx.doi.org/10.2174/2213335602666150706182206
DOI https://dx.doi.org/10.2174/2213335602666150706182206 |
Print ISSN 2213-3356 |
Publisher Name Bentham Science Publisher |
Online ISSN 2213-3364 |
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