From Classical to High Throughput Screening Methods for Feruloyl Esterases: A Review

Title:From Classical to High Throughput Screening Methods for Feruloyl Esterases: A Review

Volume: 19 Issue: 8

Author(s): Lorena Ramírez-Velasco, Mariana Armendáriz-Ruiz, Jorge Alberto Rodríguez-González, Marcelo Müller-Santos, Ali Asaff-Torres and Juan Carlos Mateos-Díaz

Affiliation:

Keywords: Chromogenic substrates, classical methods, ferulic acid, feruloyl esterases, high throughput screening, hydroxycinnamic esters.

Abstract: Feruloyl esterases (FAEs) are a diverse group of hydrolases widely distributed in plants and microorganisms which catalyzes the cleavage and formation of ester bonds between plant cell wall polysaccharides and phenolic acids. FAEs have gained importance in biofuel, medicine and food industries due to their capability of acting on a large range of substrates for cleaving ester bonds and synthesizing highadded value molecules through esterification and transesterification reactions. During the past two decades extensive studies have been carried out on the production, characterization and classification of FAEs, however only a few reports of suitable High Throughput Screening assays for this kind of enzymes have been reported. This review is focused on a concise but complete revision of classical to High Throughput Screening methods for FAEs, highlighting its advantages and disadvantages, and finally suggesting future perspectives for this important research field.

Cite this article as:

Ramírez-Velasco Lorena, Armendáriz-Ruiz Mariana, Rodríguez-González Alberto Jorge, Müller-Santos Marcelo, Asaff-Torres Ali and Mateos-Díaz Carlos Juan, From Classical to High Throughput Screening Methods for Feruloyl Esterases: A Review, Combinatorial Chemistry & High Throughput Screening 2016; 19 (8) . https://dx.doi.org/10.2174/1386207319666151110154722