Secretome Analysis of a Pseudomonas aeruginosa Strain Grown Under High Alkane Stress

Title:Secretome Analysis of a Pseudomonas aeruginosa Strain Grown Under High Alkane Stress

Volume: 12 Issue: 3

Author(s): R. Hemamalini and Sunil Kumar Khare

Affiliation:

Keywords: Elastase, endopeptidase, OprF, GroEL, superoxide dismutase, solvent tolerance.

Abstract: The effect of alkanes, viz. n-hexane, n-heptane and n-octane on the extracellular proteome and secretome of a solvent tolerant Pseudomonas aeruginosa has been described. Superoxide dismutase, GroEL, A-type flagellin and the porin OprF were altered significantly. The presence of these proteins in the extracellular proteome of solvent-grown cells indicates their role in the cellular adaptation of the strain to a high alkane environment. Up regulation of superoxide dismutase indicates creation of oxidative stress for the bacterial cell in an alkane-rich environment. This secretome analysis is useful for gaining a better understanding of the solvent mediated oxidative stress and the role of superoxide dismutase and GroEL expression as possible markers of solvent tolerance.

Cite this article as:

Hemamalini R. and Kumar Khare Sunil, Secretome Analysis of a Pseudomonas aeruginosa Strain Grown Under High Alkane Stress, Current Proteomics 2015; 12 (3) . https://dx.doi.org/10.2174/157016461203151120094228