Ciprofloxacin Stress Proteome of the Extended-Spectrum β-lactamase Producing Escherichia coli from Slaughtered Pigs

Title:Ciprofloxacin Stress Proteome of the Extended-Spectrum β-lactamase Producing Escherichia coli from Slaughtered Pigs

Volume: 13 Issue: 4

Author(s): Sonia Ramos, Ingrid Chafsey, Michel Hebraud, Margarida Sousa, Patricia Poeta and Gilberto Igrejas

Affiliation:

Keywords: Antibiotic resistance, ciprofloxacin, ESBL, Escherichia coli, proteomics.

Abstract: Background: Escherichia coli with its large range of pathologies is a major cause of human morbidity and mortality around the world. The increasing prevalence of bacteria resistant to even the most current arsenal of antibiotics is a serious concern for public health globally. The resistant bacteria which cause human infection are thought to emerge in food and animals.

Objective: Our goal was to study the proteome of an extended-spectrum β-lactamase (ESBL)- producing Escherichia coli, SU03, strain recovered from faecal samples of pigs slaughtered for human consumption.

Method: A full proteomic survey of this strain was made by two-dimensional electrophoresis identifying proteins by MALDI-TOF/MS.

Results: This strain exhibited ciprofloxacin resistance associated with mutations in type II topoisomerase structural gene GyrA (Ser83Leu + Asp87Asn) and ParC (Ser80Ile). We studied how the proteome of this strain responded to stress by applying double the minimum inhibitory concentration of ciprofloxacin. The hydrolase L-asparaginase was overexpressed when SU03 was cultured with double the minimum inhibitory concentration of ciprofloxacin.

Conclusion: The abundance of this hydrolase may lead to a diverse secondary response by influencing in the production of other proteins or directly mediating ciprofloxacin resistance. Further research should determine how this enzyme contributes to ciprofloxacin resistance.

Cite this article as:

Ramos Sonia, Chafsey Ingrid, Hebraud Michel, Sousa Margarida, Poeta Patricia and Igrejas Gilberto, Ciprofloxacin Stress Proteome of the Extended-Spectrum β-lactamase Producing Escherichia coli from Slaughtered Pigs, Current Proteomics 2016; 13 (4) . https://dx.doi.org/10.2174/1570164613666161018144215

DOI https://dx.doi.org/10.2174/1570164613666161018144215	Print ISSN 1570-1646
Publisher Name Bentham Science Publisher	Online ISSN 1875-6247