Abstract
In the present study, polyethylenimine (PEI, 1800 Da) and liposome were combined in order to improve gene expression. A new gene delivery system (polycationic liposome) was developed by modification of liposomes with lipoploymers constructed from acrylate or bromoalkane derivatives. The polycationic liposome-plasmid DNA (pDNA) complexes were characterized for their size, zeta potential and ability for DNA condensation. Luciferase reporter gene was used for the determination of transfection efficiency in Neuro2A cells. While mean particle size of prepared vectors ranged from 75 to 520 nm, the zeta potential varied from 11-35 mV. Transfection activity of selected non-viral vectors was higher than that of PEI 1800 Da and PEI 25 KDa. The transfection activity of polycationic liposomes was reduced after replacement of bromoalkane derivatives by acrylates in the structure of lipopolymers. Furthermore, gene carriers described in this study showed low cytotoxicity. The results show that inclusion of hydrophobic PEI derivatives in liposome structure can improve the transfection efficiency but the lipopolymer structure determines the gene expression efficiency of vectors
Keywords: Lipopolymer, liposomes, non-viral gene delivery, polyethylenimine.
Current Nanoscience
Title:The Effect of Lipopolymer Structure on the Transfection Efficiency of Hydrophobic Polyethylenimine-based Cationic Nanoliposomes
Volume: 8 Issue: 5
Author(s): Mohammad Ramezani, Bizhan Malaekeh-Nikouei, Susan Malekzadeh, Mohammad Reza Baghayeripour and Mohammad Malaekeh-Nikouei
Affiliation:
Keywords: Lipopolymer, liposomes, non-viral gene delivery, polyethylenimine.
Abstract: In the present study, polyethylenimine (PEI, 1800 Da) and liposome were combined in order to improve gene expression. A new gene delivery system (polycationic liposome) was developed by modification of liposomes with lipoploymers constructed from acrylate or bromoalkane derivatives. The polycationic liposome-plasmid DNA (pDNA) complexes were characterized for their size, zeta potential and ability for DNA condensation. Luciferase reporter gene was used for the determination of transfection efficiency in Neuro2A cells. While mean particle size of prepared vectors ranged from 75 to 520 nm, the zeta potential varied from 11-35 mV. Transfection activity of selected non-viral vectors was higher than that of PEI 1800 Da and PEI 25 KDa. The transfection activity of polycationic liposomes was reduced after replacement of bromoalkane derivatives by acrylates in the structure of lipopolymers. Furthermore, gene carriers described in this study showed low cytotoxicity. The results show that inclusion of hydrophobic PEI derivatives in liposome structure can improve the transfection efficiency but the lipopolymer structure determines the gene expression efficiency of vectors
Export Options
About this article
Cite this article as:
Ramezani Mohammad, Malaekeh-Nikouei Bizhan, Malekzadeh Susan, Reza Baghayeripour Mohammad and Malaekeh-Nikouei Mohammad, The Effect of Lipopolymer Structure on the Transfection Efficiency of Hydrophobic Polyethylenimine-based Cationic Nanoliposomes, Current Nanoscience 2012; 8 (5) . https://dx.doi.org/10.2174/157341312802884535
DOI https://dx.doi.org/10.2174/157341312802884535 |
Print ISSN 1573-4137 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6786 |
Call for Papers in Thematic Issues
Role of nanomaterials in fabrication of coatings, Machining and Joining
The application of nanoscience has brought about a revolution in the field of mechanical engineering by providing novel materials, boosting manufacturing processes, and generating cutting-edge products. The purpose of this special issue is to investigate the enormous impact that nanoscience has had on mechanical engineering, with a particular emphasis on ...read more
Advanced Inorganic Nanocomposites and Their Emerging Applications
This special issue collection will highlight developments on the recent trends about the synthesis of metal oxides, nanoclusters, biomaterials, 2D nanomaterials, nanocrystals, nanocomposites, etc. and their applications in electrochemical systems, tissue regeneration, energy storage and harvesting, sensors, etc. The novelty of the methods in the chemical synthesis and their characterizations, ...read more
Applicability of Nanotechnology for Performance Enhancement of Clean Energy Technologies
Population growth, industrialization, and improvement in living quality would lead to further increase in energy demand in near future. Regarding the disadvantages of fossil fuels such as fluctuations in their price, high emissions of greenhouse gases and restriction of their sources, it is crucial to use and exploit alternative energy ...read more
Graphene and 2D Materials for Energy Storage and Conversion
This thematic issue will discuss the recent advances in graphene-based nanomaterials for different energy technologies. Graphene possesses a high surface area, and stable structure and exhibits many interesting electronic, optical, and mechanical properties due to its 2D crystal structure. Graphene is of both fundamental interest and suitable for a wide ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Lipid Nanoparticles to Deliver miRNA in Cancer
Current Pharmaceutical Biotechnology The Association Between Alcohol Use and the Progression of Alzheimer’s Disease
Current Alzheimer Research Critical Questions for Preclinical Trials on Safety and Efficacy of Vascular Endothelial Growth Factor-Based Therapeutic Angiogenesis for Ischemic Stroke
CNS & Neurological Disorders - Drug Targets Glaucoma Pathogenesis and Neurotrophins: Focus on the Molecular and Genetic Basis for Therapeutic Prospects
Current Neuropharmacology Cyclic AMP Enhancers and Aβ Oligomerization Blockers as Potential Therapeutic Agents in Alzheimers Disease
Current Alzheimer Research Patented Small Molecules Used for Reprogramming
Recent Patents on Regenerative Medicine The Neurotrophins and Their Role in Alzheimers Disease
Current Neuropharmacology Antioxidant, Pro-Oxidant and Other Biological Activities of Sesquiterpenes
Current Topics in Medicinal Chemistry Recent Trends in Tubulin-Binding Combretastatin A-4 Analogs for Anticancer Drug Development
Current Medicinal Chemistry Monoamine Oxidase Inhibitors as Neuroprotective Agents in Age-Dependent Neurodegenerative Disorders
Current Pharmaceutical Design Tumor-Targeted Inhibition by a Novel Strategy - Mimoretrovirus Expressing siRNA Targeting the Pokemon Gene
Current Cancer Drug Targets Therapeutic Potential of microRNA Modulation in Pulmonary Arterial Hypertension
Current Vascular Pharmacology Current Evaluation of the Millennium Phytomedicine- Ginseng (II): Collected Chemical Entities, Modern Pharmacology, and Clinical Applications Emanated from Traditional Chinese Medicine
Current Medicinal Chemistry IAP Proteins Antagonist: An Introduction and Chemistry of Smac Mimetics under Clinical Development
Current Medicinal Chemistry Resveratrol: A New Potential Therapeutic Agent for Melanoma?
Current Medicinal Chemistry Antineoplastic Potential of Medicinal Plants
Recent Patents on Biotechnology Biomarker Assessment in Nutritional Modulation of Oxidative Stress-Induced Cancer Development by Lipid-Related Bioactive Molecules
Recent Patents on Anti-Cancer Drug Discovery The Exploitation of Toll-like Receptor 3 Signaling in Cancer Therapy
Current Pharmaceutical Design Compartmentalized Platforms for Neuro-Pharmacological Research
Current Neuropharmacology An Overview of the α4β1 Integrin and the Potential Therapeutic Role of its Antagonists
Current Medicinal Chemistry