Abstract
Diblock copolymers of poly(ethylene glycol) (PEG) and poly(ε-caprolactone) (PCL) bearing a biotin ligand, were self-assembled into micelles. Superparamagnetic iron oxide (SPIO) nanoparticles and an anticancer drug methotrexate (MTX) were coencapsulated within the micelles less than 100 nm in diameters.
The preparation process was optimized by a systematic multi-objective-optimization approach in terms of the encapsulation ratio (ER %, Y1), drug-loading efficiency (DL %, Y2) and the percentage of MTX precipitated from the drug-loaded mixed micelles after 30min and 6h incubation at 37ºC (MTX precipitated %, Y3 and Y4) of the resulting nanocarriers. A combination of the stirring rate, ultrasonic power and drug/polymer ratio, accounted for nearly 79% of the variation in drug-loading efficiency. The final entrapment ratio, loading efficiency and MTX % precipitated corresponding to the optimal conditions were 70.938, 5.665 and 6.885 respectively.
Moreover, in vitro release behavior of MTX was also investigated. The result shows, MTX is physically entrapped inside B-PEG-PCL self-aggregated nanoparticlesis.
Keywords: Biotin, Drug delivery, Nano micelle, Optimization, Plackett-Burman, SoCl2.
Letters in Drug Design & Discovery
Title:Biotin-Encoded and Fe3O4-Loaded Polymeric Nano Micelles: Preparation, Optimization and In Vitro Characterization
Volume: 10 Issue: 10
Author(s): Mohammad Hossein Ahmadi Azqhandi, Mohammad Reza Khanmohammadi, Bahman Vasheghani Farahani and Reza Najafipour
Affiliation:
Keywords: Biotin, Drug delivery, Nano micelle, Optimization, Plackett-Burman, SoCl2.
Abstract: Diblock copolymers of poly(ethylene glycol) (PEG) and poly(ε-caprolactone) (PCL) bearing a biotin ligand, were self-assembled into micelles. Superparamagnetic iron oxide (SPIO) nanoparticles and an anticancer drug methotrexate (MTX) were coencapsulated within the micelles less than 100 nm in diameters.
The preparation process was optimized by a systematic multi-objective-optimization approach in terms of the encapsulation ratio (ER %, Y1), drug-loading efficiency (DL %, Y2) and the percentage of MTX precipitated from the drug-loaded mixed micelles after 30min and 6h incubation at 37ºC (MTX precipitated %, Y3 and Y4) of the resulting nanocarriers. A combination of the stirring rate, ultrasonic power and drug/polymer ratio, accounted for nearly 79% of the variation in drug-loading efficiency. The final entrapment ratio, loading efficiency and MTX % precipitated corresponding to the optimal conditions were 70.938, 5.665 and 6.885 respectively.
Moreover, in vitro release behavior of MTX was also investigated. The result shows, MTX is physically entrapped inside B-PEG-PCL self-aggregated nanoparticlesis.
Export Options
About this article
Cite this article as:
Azqhandi Hossein Ahmadi Mohammad, Khanmohammadi Reza Mohammad, Farahani Vasheghani Bahman and Najafipour Reza, Biotin-Encoded and Fe3O4-Loaded Polymeric Nano Micelles: Preparation, Optimization and In Vitro Characterization, Letters in Drug Design & Discovery 2013; 10 (10) . https://dx.doi.org/10.2174/15701808113109990033
DOI https://dx.doi.org/10.2174/15701808113109990033 |
Print ISSN 1570-1808 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-628X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
mTOR in Growth and Protection of Hypertrophying Myocardium
Cardiovascular & Hematological Agents in Medicinal Chemistry Toll Like Receptors Signaling Pathways as a Target for Therapeutic Interventions
Current Signal Transduction Therapy Altering the Tropism of Lentiviral Vectors through Pseudotyping
Current Gene Therapy Therapeutic Targeting of G-Protein Coupled Receptor-Mediated Epidermal Growth Factor Receptor Transactivation in Human Glioma Brain Tumors
Mini-Reviews in Medicinal Chemistry Pyrimidine Nucleosides in Molecular PET Imaging of Tumor Proliferation
Current Medicinal Chemistry Mechano-Regulation of Alternative Splicing
Current Genomics Recent Advances in Application of Poly-Epsilon-Caprolactone and its Derivative Copolymers for Controlled Release of Anti-Tumor Drugs
Current Cancer Drug Targets A Novel Method for Screening of Anti-Cancer Drugs: Availability of Screening in Acidic Medium
Recent Patents on Biomedical Engineering (Discontinued) Perspectives of Protein Kinase C (PKC) Inhibitors as Anti-Cancer Agents
Mini-Reviews in Medicinal Chemistry Mitochondria-Mediated Oxidative Stress: Old Target for New Drugs
Current Medicinal Chemistry NAD+ Metabolism and NAD+-Dependent Enzymes: Promising Therapeutic Targets for Neurological Diseases
Current Drug Targets Therapeutic Potential of Curcumin in the Treatment of Glioblastoma Multiforme
Current Pharmaceutical Design KCa3.1 Channel Modulators as Potential Therapeutic Compounds for Glioblastoma
Current Neuropharmacology Procyanidin B2 3,3″-di-O-gallate Inhibits Endothelial Cells Growth and Motility by Targeting VEGFR2 and Integrin Signaling Pathways
Current Cancer Drug Targets Search and Rescue: Identification of Cannabinoid Actions Relevant for Neuronal Survival and Protection
Current Neuropharmacology Gene Therapy and Biologic Therapy with Interleukin?4
Current Gene Therapy The Use of the Zebrafish Model to Aid in Drug Discovery and Target Validation
Current Topics in Medicinal Chemistry A Role for Calcineurin in Alzheimers Disease
Current Neuropharmacology Uridine Function in the Central Nervous System
Current Topics in Medicinal Chemistry Lectin-Carbohydrate Interactions: Implications for the Development of New Anticancer Agents
Current Medicinal Chemistry