Abstract
Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune defense system of many living organisms varying from prokaryotes to eukaryotes, including humans. Due to their broad-spectrum activity and low level of induced resistance, these short aminoacid sequences represent a novel class of potential antimicrobial agents. Besides the development of anti-bacterial drugs, AMPs constitute ideal molecular models for the design of molecules with wide-ranging nanomedical applications, such as anti-tumorigenic agents and pharmacological tools to cure channelopaties. Several techniques are currently used to shed light on the mechanisms of action of AMPs, ranging from the characterization of the interaction between peptides and biomimetic membranes and/or intracellular targets, to the study of AMPs effects on pathogens, living cells and tissues. Comprehensive and multiscale studies are crucial to design new AMPs and to identify molecules that can boost their activity. In this minireview we summarize the most recent achievements in AMP-characterization, with a special emphasis on the integration of biophysical approaches, which can synergistically help to bridge the gap between in vitro and ex vivo investigations.
Keywords: Antimicrobial peptides, membrane permeabilization, peptide-membrane interaction, pore-forming peptides, surface plasmon resonance, whole-cell patch clamp.
Current Protein & Peptide Science
Title:Biophysical Characterization of Antimicrobial Peptides Activity: From In Vitro to Ex Vivo Techniques
Volume: 14 Issue: 7
Author(s): Marco Aquila, Mascia Benedusi and Daniele Dell’Orco
Affiliation:
Keywords: Antimicrobial peptides, membrane permeabilization, peptide-membrane interaction, pore-forming peptides, surface plasmon resonance, whole-cell patch clamp.
Abstract: Antimicrobial peptides (AMPs) are evolutionarily conserved components of the innate immune defense system of many living organisms varying from prokaryotes to eukaryotes, including humans. Due to their broad-spectrum activity and low level of induced resistance, these short aminoacid sequences represent a novel class of potential antimicrobial agents. Besides the development of anti-bacterial drugs, AMPs constitute ideal molecular models for the design of molecules with wide-ranging nanomedical applications, such as anti-tumorigenic agents and pharmacological tools to cure channelopaties. Several techniques are currently used to shed light on the mechanisms of action of AMPs, ranging from the characterization of the interaction between peptides and biomimetic membranes and/or intracellular targets, to the study of AMPs effects on pathogens, living cells and tissues. Comprehensive and multiscale studies are crucial to design new AMPs and to identify molecules that can boost their activity. In this minireview we summarize the most recent achievements in AMP-characterization, with a special emphasis on the integration of biophysical approaches, which can synergistically help to bridge the gap between in vitro and ex vivo investigations.
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Cite this article as:
Aquila Marco, Benedusi Mascia and Dell’Orco Daniele, Biophysical Characterization of Antimicrobial Peptides Activity: From In Vitro to Ex Vivo Techniques, Current Protein & Peptide Science 2013; 14 (7) . https://dx.doi.org/10.2174/13892037113146660088
DOI https://dx.doi.org/10.2174/13892037113146660088 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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