Abstract
Metabolic Engineering (ME) provides the know-how for the reconstruction of microorganisms, in order to provide higher production rates of biotechnological products and increase their potential application in several industries, particularly those pharmaceutical, food and environmental. However, microbial metabolic cell reconstruction has limitations. Metabolic Control Analysis (MCA) allows the evaluation of the reliability of the changes performed by ME, emphasizing the importance of the whole pathways rather than individual pathway reactions resulting from simple flux analysis. This led to an increased emphasis on the regulatory structure of the network. The use of MCA becomes indispensable to quantify metabolic parameters, particularly those related with direct genetic modifications. However, this type of analysis is not a common practice. In the present study MCA is exemplified as a tool of ME, being demonstrated its practical application in drug delivery and in the production of three relevant biotechnological products (penicillin V, llysine and glycerol).
Keywords: Control coefficient, drug delivery, enzyme, glycerol, kinetics, l-lysine, metabolic control analysis, penicillin V.
Current Bioinformatics
Title:Metabolic Control Analysis and its Applications
Volume: 9 Issue: 5
Author(s): Ana Meireles and Manuel Simoes
Affiliation:
Keywords: Control coefficient, drug delivery, enzyme, glycerol, kinetics, l-lysine, metabolic control analysis, penicillin V.
Abstract: Metabolic Engineering (ME) provides the know-how for the reconstruction of microorganisms, in order to provide higher production rates of biotechnological products and increase their potential application in several industries, particularly those pharmaceutical, food and environmental. However, microbial metabolic cell reconstruction has limitations. Metabolic Control Analysis (MCA) allows the evaluation of the reliability of the changes performed by ME, emphasizing the importance of the whole pathways rather than individual pathway reactions resulting from simple flux analysis. This led to an increased emphasis on the regulatory structure of the network. The use of MCA becomes indispensable to quantify metabolic parameters, particularly those related with direct genetic modifications. However, this type of analysis is not a common practice. In the present study MCA is exemplified as a tool of ME, being demonstrated its practical application in drug delivery and in the production of three relevant biotechnological products (penicillin V, llysine and glycerol).
Export Options
About this article
Cite this article as:
Meireles Ana and Simoes Manuel, Metabolic Control Analysis and its Applications, Current Bioinformatics 2014; 9 (5) . https://dx.doi.org/10.2174/1574893609666140515230435
DOI https://dx.doi.org/10.2174/1574893609666140515230435 |
Print ISSN 1574-8936 |
Publisher Name Bentham Science Publisher |
Online ISSN 2212-392X |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Shoulder Manifestations of Diabetes Mellitus
Current Diabetes Reviews Genetic Predisposition in NAFLD and NASH: Impact on Severity of Liver Disease and Response to Treatment
Current Pharmaceutical Design Peripheral Arterial Disease in Persons with Diabetic Foot Ulceration: a Current Comprehensive Overview
Current Diabetes Reviews Inflammation in Atherosclerosis: A New Therapeutic Target
Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents BCR-ABL Transcript Level as Compared to LDH and Uric Acid Among Chronic Myeloid Leukemic Patients
Recent Patents on Anti-Cancer Drug Discovery Molecular Linkages Between Diabetes and Alzheimer's Disease: Current Scenario and Future Prospects
CNS & Neurological Disorders - Drug Targets Does It Make Sense that Diabetes is Reciprocally Associated with Periodontal Disease?
Endocrine, Metabolic & Immune Disorders - Drug Targets The Endothelial-Mesenchymal Transition (EndMT) and Tissue Regeneration
Current Stem Cell Research & Therapy Protection by Natural Honey Against Hyperhomocysteinemia in Rats
Vascular Disease Prevention (Discontinued) Recent Insights into the Role of Prostanoids in Atherosclerotic Vascular Disease
Current Vascular Pharmacology Classical and Innovative Insulin Sensitizing Drugs for the Prevention and Treatment of NAFLD
Current Pharmaceutical Design Antioxidant Activity of Uva di Troia Canosina: Comparison of Two Extraction Methods
Clinical Immunology, Endocrine & Metabolic Drugs (Discontinued) Patent Selections
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery Prevention of Gestational Diabetes Mellitus (GDM) and Probiotics: Mechanism of Action: A Review
Current Diabetes Reviews Hepatic Elimination of Drugs in Gestational Diabetes
Current Clinical Pharmacology An Insight on the Leading HIV Entry Inhibitors
Recent Patents on Anti-Infective Drug Discovery Role of Peroxisome Proliferator-Activated Receptor Gamma (PPARγ) in Different Disease States: Recent Updates
Current Medicinal Chemistry Cardiovascular Complications of Obesity
Current Respiratory Medicine Reviews How to Advise Aspirin Use in Patients Who Need NSAIDs
Current Pharmaceutical Design Human postnatal Mesenchymal Stem Cell Derived Islets as a Model for Diabetes Research
Current Diabetes Reviews