Abstract
The N-end rule pathway is a conserved targeted proteolytic process observed in organisms ranging from eubacteria to mammals. The N-end rule relates the metabolic stability of a protein to its N-terminal amino acid residue. The identity of the N-terminal amino acid residue is a primary degradation signal, often referred to as an N-degron, which is recognized by the components of the N-end rule when it is a destabilizing N-terminus. N-degrons may be exposed by non-processive proteolytic cleavages or by post-translational modifications. One modification is the post-translational addition of amino acids to the N-termini of proteins, a reaction catalyzed by aminoacyl-tRNA protein transferases. The aminoacyl-tRNA protein transferase in eubacteria like Escherichia coli is L/F transferase. Recent investigations have reported unexpected observations regarding the L/F transferase catalytic mechanism and its mechanisms of substrate recognition. Additionally, recent proteome-wide identification of putative in vivo substrates facilitates hypothesis into the yet elusive biological functions of the prokaryotic N-end rule pathway. Here we summarize the recent findings on the molecular mechanisms of catalysis and substrate recognition by the E. coli L/F transferase in the prokaryotic N-end rule pathway.
Keywords: aa-tRNA, Dupli-GNAT superfamily, L/F transferase, N-end rule, non-ribosomal peptide bond formation, posttranslational addition of amino acid.
Current Protein & Peptide Science
Title:The Molecular Basis for the Post-Translational Addition of Amino Acids by L/F Transferase in the N-End Rule Pathway
Volume: 16 Issue: 2
Author(s): Angela Wai S. Fung and Richard P. Fahlman
Affiliation:
Keywords: aa-tRNA, Dupli-GNAT superfamily, L/F transferase, N-end rule, non-ribosomal peptide bond formation, posttranslational addition of amino acid.
Abstract: The N-end rule pathway is a conserved targeted proteolytic process observed in organisms ranging from eubacteria to mammals. The N-end rule relates the metabolic stability of a protein to its N-terminal amino acid residue. The identity of the N-terminal amino acid residue is a primary degradation signal, often referred to as an N-degron, which is recognized by the components of the N-end rule when it is a destabilizing N-terminus. N-degrons may be exposed by non-processive proteolytic cleavages or by post-translational modifications. One modification is the post-translational addition of amino acids to the N-termini of proteins, a reaction catalyzed by aminoacyl-tRNA protein transferases. The aminoacyl-tRNA protein transferase in eubacteria like Escherichia coli is L/F transferase. Recent investigations have reported unexpected observations regarding the L/F transferase catalytic mechanism and its mechanisms of substrate recognition. Additionally, recent proteome-wide identification of putative in vivo substrates facilitates hypothesis into the yet elusive biological functions of the prokaryotic N-end rule pathway. Here we summarize the recent findings on the molecular mechanisms of catalysis and substrate recognition by the E. coli L/F transferase in the prokaryotic N-end rule pathway.
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Cite this article as:
S. Fung Wai Angela and Fahlman P. Richard, The Molecular Basis for the Post-Translational Addition of Amino Acids by L/F Transferase in the N-End Rule Pathway, Current Protein & Peptide Science 2015; 16 (2) . https://dx.doi.org/10.2174/1389203716666150112095726
DOI https://dx.doi.org/10.2174/1389203716666150112095726 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
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