Prediction Biomarkers Associated with Lymph Node Metastasis and Prognosis were Identified in Papillary Thyroid Carcinoma via Integrated Bioinformatics Analysis

Title:Prediction Biomarkers Associated with Lymph Node Metastasis and Prognosis were Identified in Papillary Thyroid Carcinoma via Integrated Bioinformatics Analysis

Volume: 24 Issue: 9

Author(s): Siyuan Gan*, Ruifang Ye*, Yanping Ha*, Yinghuan Xiong*, Rujia Li, Xiaoqing Di, Zhenning Zou, Yanqin Sun and Zhi Zhang

Affiliation:

• Department of Pathology, Guangdong Medical University, Zhanjiang,China

• Department of Pathology, Guangdong Medical University, Zhanjiang,China

• Department of Pathology, Guangdong Medical University, Zhanjiang,China

• Department of Pathology, The Affiliated Hospital of Guangdong Medical University, Zhanjiang,China

Keywords: Papillary thyroid carcinoma, lymph node metastasis, prognosis, bioinformatics, genes, PPI.

Abstract: Background: Prediction biomarkers associated with prognosis and lymph node metastasis (LNM) in papillary thyroid carcinoma (PTC) are needed to facilitate clinicians in choosing appropriate therapies.

Objective: We hope to identify key genes associated with LNM and prognosis in PTC.

Methods: GSE29265, GSE33630, GSE3467, GSE3678 and GSE58545 gene expression profiles were acquired from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) between PTC tissues and normal thyroid tissues were selected with the GEO2R tool, and common DEGs among the five datasets were integrated with Venn software online. A proteinprotein interaction (PPI) network of the common DEGs was visualized. We analyzed the PPI network and determined core genes using the Cytoscape software. Furthermore, we employed UALCAN to verify the expression and promoter methylation status of the core genes in thyroid carcinoma (THCA). Additionally, the Kaplan–Meier plotter online tool was used to analyze the relationship between overall survival and core gene expressions in THCA.

Results: TNS3, DUSP6, DUSP4 and PTPRE were identified as core genes. Expression of these 4 genes and the promoter methylation status of DUSP4 and PTPRE were strongly associated with LNM (P<0.05). High expression of 3 genes (DUSP6, DUSP4 and PTPRE) was related to a significantly better survival than low expression of the 3 genes in THCA. In contrast, high TNS3 expression was related to significantly worse survival (P<0.05).

Conclusion: TNS3, DUSP6, DUSP4, PTPRE and DUSP4 and PTPRE promoter methylation status might be useful predictive biomarkers of LNM in PTC. Additionally, these genes may be prognostic biomarkers in PTC.

Cite this article as:

Gan Siyuan *, Ye Ruifang *, Ha Yanping *, Xiong Yinghuan *, Li Rujia , Di Xiaoqing , Zou Zhenning , Sun Yanqin and Zhang Zhi , Prediction Biomarkers Associated with Lymph Node Metastasis and Prognosis were Identified in Papillary Thyroid Carcinoma via Integrated Bioinformatics Analysis, Combinatorial Chemistry & High Throughput Screening 2021; 24 (9) . https://dx.doi.org/10.2174/1386207323999201103205745

DOI https://dx.doi.org/10.2174/1386207323999201103205745	Print ISSN 1386-2073
Publisher Name Bentham Science Publisher	Online ISSN 1875-5402