Abstract
The lung represents an important target for gene therapy: for correction of genetic abnormalities such as cystic fibrosis, for lung cancer therapy, and for vaccination. Genes in the form of expression plasmids can be delivered both by the intravenous route and via the airways. So-called “naked” DNA can be delivered by both of these methods, but gene expression is low. Successful delivery is usually accomplished by complexing the DNA with cationic lipids or with polycations. This review will discuss the efficacy of delivery for particular purposes by various methods and complexing agents, as well as issues of biodistribution, inflammatory reactions, and improvements in formulations. Non-viral gene delivery to the lung has a long history of development, and it is now poised to represent a significant addition to the medical arsenal.
Keywords: inflammatory diseases, gene therapy, immunization, plasmid dna, lipoplexes, expression
Current Gene Therapy
Title: Non-Viral Gene Delivery to the Lungs
Volume: 5 Issue: 2
Author(s): Berma M. Kinsey, Charles L. Densmore and Frank M. Orson
Affiliation:
Keywords: inflammatory diseases, gene therapy, immunization, plasmid dna, lipoplexes, expression
Abstract: The lung represents an important target for gene therapy: for correction of genetic abnormalities such as cystic fibrosis, for lung cancer therapy, and for vaccination. Genes in the form of expression plasmids can be delivered both by the intravenous route and via the airways. So-called “naked” DNA can be delivered by both of these methods, but gene expression is low. Successful delivery is usually accomplished by complexing the DNA with cationic lipids or with polycations. This review will discuss the efficacy of delivery for particular purposes by various methods and complexing agents, as well as issues of biodistribution, inflammatory reactions, and improvements in formulations. Non-viral gene delivery to the lung has a long history of development, and it is now poised to represent a significant addition to the medical arsenal.
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Cite this article as:
Kinsey M. Berma, Densmore L. Charles and Orson M. Frank, Non-Viral Gene Delivery to the Lungs, Current Gene Therapy 2005; 5 (2) . https://dx.doi.org/10.2174/1566523053544254
DOI https://dx.doi.org/10.2174/1566523053544254 |
Print ISSN 1566-5232 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5631 |
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