Abstract
To facilitate automated patch clamp measurements of ion channels in cells, the development of an all-glass Chiptip pipette is reported that may be combined with the previously described Flip-the-Tip technology. A single measurement requires less than 50 cells, and the addition of drugs for screening can be limited to very low volumes down to 1 μL. This apparatus is suitable for the study small cells, subcellular organelles and bacteria.
Keywords: ligand gated channel, voltage gated channel, membrane protein, glass micropipettes, automated patch clamp, Ion channel
Combinatorial Chemistry & High Throughput Screening
Title: The Chiptip: A Novel Tool for Automated Patch Clamp
Volume: 12 Issue: 1
Author(s): Andjelko Golubovic, Martina Knirsch, Uwe Czubayko, Alexander Hummer, Albrecht Lepple-Wienhues and Dirck Lassen
Affiliation:
Keywords: ligand gated channel, voltage gated channel, membrane protein, glass micropipettes, automated patch clamp, Ion channel
Abstract: To facilitate automated patch clamp measurements of ion channels in cells, the development of an all-glass Chiptip pipette is reported that may be combined with the previously described Flip-the-Tip technology. A single measurement requires less than 50 cells, and the addition of drugs for screening can be limited to very low volumes down to 1 μL. This apparatus is suitable for the study small cells, subcellular organelles and bacteria.
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Cite this article as:
Golubovic Andjelko, Knirsch Martina, Czubayko Uwe, Hummer Alexander, Lepple-Wienhues Albrecht and Lassen Dirck, The Chiptip: A Novel Tool for Automated Patch Clamp, Combinatorial Chemistry & High Throughput Screening 2009; 12 (1) . https://dx.doi.org/10.2174/138620709787047984
DOI https://dx.doi.org/10.2174/138620709787047984 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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