Abstract
Replication deficient adenovirus vectors are frequently used tools for the delivery of transgenes in vitro and in vivo. In addition, several therapeutic products based on adenovirus are under clinical development. This review outlines adenovirus vector production discussing different vector types, available production cell lines and state of the art of production process development and purification.
Keywords: Adenovirus, adenovirus vector, production, production cell lines, process development, purification, oncolytic Ad vectors, E1Ad, hAd5, E2A, DNA-binding protein, E2B (polymerase and preterminal protein, E1, E2, E3 and E4, pol, pTP, (HC-Ad) vector, human embryonic kidney (HEK) cells, keratinocytes, hepatocytes, urothelial cells, plasmid expressing, human embryonic retinoblasts, RCA, phosphoglycerate kinase (pgk) promoter, PER.C6, helper-dependent E1-positive particles (HDEPs), E1/pIX genes, N52.E6, A549 cells, 293, 911, NCL, GH329, Ac51 Ac139, SL0003, cell concentration at infection (CCI), multiplicity of infection (MOI), Cytodex-1, Cytodex-3 microcarriers, FLPe, HDAd, TOI, time of harvest (TOH), TCA cycle, Metabolic flux analysis, DO, pCO2, polysorbate-80, Pluronic-F68, plaque-forming unit (PFU), tissue culture infectious dose 50 (TCID50), EM, high performance liquid chromatography (HPLC), Adenovirus Reference Material Working Group (ARMWG), GFP-expressing AdV, Canji, Schering, Aventis, Introgen, Merck, AEX (Fractogel DEAE, AEX (Toyopearl SuperQ, immobilized metal affinity chromatography (IMAC)
Current Gene Therapy
Title: Adenovirus Vector Production and Purification
Volume: 10 Issue: 6
Author(s): Ana Carina Silva, Cristina Peixoto, Tanja Lucas, Claudia Kuppers, Pedro E. Cruz, Paula M. Alves and Stefan Kochanek
Affiliation:
Keywords: Adenovirus, adenovirus vector, production, production cell lines, process development, purification, oncolytic Ad vectors, E1Ad, hAd5, E2A, DNA-binding protein, E2B (polymerase and preterminal protein, E1, E2, E3 and E4, pol, pTP, (HC-Ad) vector, human embryonic kidney (HEK) cells, keratinocytes, hepatocytes, urothelial cells, plasmid expressing, human embryonic retinoblasts, RCA, phosphoglycerate kinase (pgk) promoter, PER.C6, helper-dependent E1-positive particles (HDEPs), E1/pIX genes, N52.E6, A549 cells, 293, 911, NCL, GH329, Ac51 Ac139, SL0003, cell concentration at infection (CCI), multiplicity of infection (MOI), Cytodex-1, Cytodex-3 microcarriers, FLPe, HDAd, TOI, time of harvest (TOH), TCA cycle, Metabolic flux analysis, DO, pCO2, polysorbate-80, Pluronic-F68, plaque-forming unit (PFU), tissue culture infectious dose 50 (TCID50), EM, high performance liquid chromatography (HPLC), Adenovirus Reference Material Working Group (ARMWG), GFP-expressing AdV, Canji, Schering, Aventis, Introgen, Merck, AEX (Fractogel DEAE, AEX (Toyopearl SuperQ, immobilized metal affinity chromatography (IMAC)
Abstract: Replication deficient adenovirus vectors are frequently used tools for the delivery of transgenes in vitro and in vivo. In addition, several therapeutic products based on adenovirus are under clinical development. This review outlines adenovirus vector production discussing different vector types, available production cell lines and state of the art of production process development and purification.
Export Options
About this article
Cite this article as:
Carina Silva Ana, Peixoto Cristina, Lucas Tanja, Kuppers Claudia, E. Cruz Pedro, M. Alves Paula and Kochanek Stefan, Adenovirus Vector Production and Purification, Current Gene Therapy 2010; 10 (6) . https://dx.doi.org/10.2174/156652310793797694
DOI https://dx.doi.org/10.2174/156652310793797694 |
Print ISSN 1566-5232 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5631 |
Call for Papers in Thematic Issues
Programmed Cell Death Genes in Oncology: Pioneering Therapeutic and Diagnostic Frontiers (BMS-CGT-2024-HT-45)
Programmed Cell Death (PCD) is recognized as a pivotal biological mechanism with far-reaching effects in the realm of cancer therapy. This complex process encompasses a variety of cell death modalities, including apoptosis, autophagic cell death, pyroptosis, and ferroptosis, each of which contributes to the intricate landscape of cancer development and ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Therapeutic Strategies to Target Multiple Kinases in Glioblastoma
Anti-Cancer Agents in Medicinal Chemistry Effects of Galbanic Acid on Proliferation, Migration, and Apoptosis of Glioblastoma Cells Through the PI3K/Akt/MTOR Signaling Pathway
Current Molecular Pharmacology Muscarinic Acetylcholine Receptors: New Potential Therapeutic Targets in Antinociception and in Cancer Therapy
Recent Patents on CNS Drug Discovery (Discontinued) Importance of Influx and Efflux Systems and Xenobiotic Metabolizing Enzymes in Intratumoral Disposition of Anticancer Agents
Current Cancer Drug Targets Interlocked Systems in Nanomedicine
Current Topics in Medicinal Chemistry FOXM1-Dependent Transcriptional Regulation of EZH2 Induces Proliferation and Progression in Prostate Cancer
Anti-Cancer Agents in Medicinal Chemistry Decorin Biology, Expression, Function and Therapy in the Cornea
Current Molecular Medicine Angiogenesis Inhibitors: Implications for Combination with Conventional Therapies
Current Pharmaceutical Design MicroRNAs in Cancer Gene Therapy: Another Look
Current Cancer Therapy Reviews Erythrocyte-cancer Hybrid Membrane-camouflaged Mesoporous Silica Nanoparticles Loaded with Gboxin for Glioma-targeting Therapy
Current Pharmaceutical Biotechnology Clinical Studies with Targeted Toxins in Malignant Glioma
Reviews on Recent Clinical Trials Exploration of the Medicinal Peptide Space
Protein & Peptide Letters The Urokinase Receptor as a Potential Target in Cancer Therapy
Current Pharmaceutical Design Cellular Immunotherapy for Neuroblastoma: A Review of Current Vaccine and Adoptive T Cell Therapeutics
Current Pharmaceutical Design The Potency of Refined Mouse Models: Implications for Clinical Trials
Current Cancer Therapy Reviews Colostral Proline-Rich Polypeptides - Immunoregulatory Properties and Prospects of Therapeutic Use in Alzheimers Disease
Current Alzheimer Research Recent Advances in the Use of Metallic Nanoparticles with Antitumoral Action - Review
Current Medicinal Chemistry Nanoliposome is a Promising Carrier of Protein and Peptide Biomolecule for the Treatment of Cancer
Anti-Cancer Agents in Medicinal Chemistry Role of Mu and Delta Opioid Receptors in Alcohol Drinking Behaviour
Current Drug Abuse Reviews Relationship with Programmed Cell Death Ligand 1 (PD-L1) and DTI Features in Brain Metastases of Non-small Cell Lung Cancer: A Preliminary Study
Current Medical Imaging