Abstract
Rapid Translation System (RTS) is a cell-free protein production system employing an enhanced Escherichia coli lysate to perform coupled in vitro transcription-translation reactions. A continuous supply of energy substrates, nucleotides and amino acids combined with the removal of by-products guarantees a high yield of protein production. The gene to express is either cloned into a plasmid vector or introduced as a PCR product amenable to automation. The main property of this alternative system to cellular expression systems is its open design allowing direct manipulation of the reaction conditions and applications that are impossible or difficult in cell-based systems. RTS offers new promising possibilities in the postgenomic era.
Keywords: rapid translation system, rts, recombinant protein
Current Protein & Peptide Science
Title: Rapid Translation System (RTS): A Promising Alternative for Recombinant Protein Production
Volume: 4 Issue: 1
Author(s): J.- M. Betton
Affiliation:
Keywords: rapid translation system, rts, recombinant protein
Abstract: Rapid Translation System (RTS) is a cell-free protein production system employing an enhanced Escherichia coli lysate to perform coupled in vitro transcription-translation reactions. A continuous supply of energy substrates, nucleotides and amino acids combined with the removal of by-products guarantees a high yield of protein production. The gene to express is either cloned into a plasmid vector or introduced as a PCR product amenable to automation. The main property of this alternative system to cellular expression systems is its open design allowing direct manipulation of the reaction conditions and applications that are impossible or difficult in cell-based systems. RTS offers new promising possibilities in the postgenomic era.
Export Options
About this article
Cite this article as:
Betton M. J.-, Rapid Translation System (RTS): A Promising Alternative for Recombinant Protein Production, Current Protein & Peptide Science 2003; 4 (1) . https://dx.doi.org/10.2174/1389203033380359
DOI https://dx.doi.org/10.2174/1389203033380359 |
Print ISSN 1389-2037 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5550 |
Call for Papers in Thematic Issues
Advancements in Proteomic and Peptidomic Approaches in Cancer Immunotherapy: Unveiling the Immune Microenvironment
The scope of this thematic issue centers on the integration of proteomic and peptidomic technologies into the field of cancer immunotherapy, with a particular emphasis on exploring the tumor immune microenvironment. This issue aims to gather contributions that illustrate the application of these advanced methodologies in unveiling the complex interplay ...read more
Artificial Intelligence for Protein Research
Protein research, essential for understanding biological processes and creating therapeutics, faces challenges due to the intricate nature of protein structures and functions. Traditional methods are limited in exploring the vast protein sequence space efficiently. Artificial intelligence (AI) and machine learning (ML) offer promising solutions by improving predictions and speeding up ...read more
Nutrition and Metabolism in Musculoskeletal Diseases
The musculoskeletal system consists mainly of cartilage, bone, muscles, tendons, connective tissue and ligaments. Balanced metabolism is of vital importance for the homeostasis of the musculoskeletal system. A series of musculoskeletal diseases (for example, sarcopenia, osteoporosis) are resulted from the dysregulated metabolism of the musculoskeletal system. Furthermore, metabolic diseases (such ...read more
Protein Folding, Aggregation and Liquid-Liquid Phase Separation
Protein folding, misfolding and aggregation remain one of the main problems of interdisciplinary science not only because many questions are still open, but also because they are important from the point of view of practical application. Protein aggregation and formation of fibrillar structures, for example, is a hallmark of a ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Estrogens and Glucocorticoid Hormones in Adipose Tissue Metabolism
Current Medicinal Chemistry Contribution of Inflammatory Processes to Nerve Cell Toxicity by Bilirubin and Efficacy of Potential Therapeutic Agents
Current Pharmaceutical Design Signs and Related Mechanisms of Ethanol Hepatotoxicity
Current Drug Abuse Reviews Isoprostanes as Biomarkers and Mediators of Oxidative Injury in Infant and Adult Central Nervous System Diseases
Current Neurovascular Research Regeneration of Pancreatic β-Cells in vivo as a Potential Therapeutic Approach for Diabetes Mellitus
Recent Patents on Endocrine, Metabolic & Immune Drug Discovery Methicillin-Sensitive <i>Staphylococcus aureus</i> Pyogenic Iliopsoas Abscesses: A Case Series from Jodhpur, India
Infectious Disorders - Drug Targets Heat Shock Proteins: Mediators of Atherosclerotic Development
Current Drug Targets Functional and Molecular Ultrasound Imaging: Concepts and Contrast Agents
Current Medicinal Chemistry Drug-Mediated Targeted Disruption of Multiple Protein Activities Through Functional Inhibition of the Hsp90 Chaperone Complex
Current Medicinal Chemistry The Blood-brain Barrier and the Outer Blood-retina Barrier
Medicinal Chemistry Reviews - Online (Discontinued) Effects of Alcohol in the Lung
Current Respiratory Medicine Reviews Platelet SERT as a Peripheral Biomarker of Serotonergic Neurotransmission in the Central Nervous System
Current Medicinal Chemistry Transporters at CNS Barrier Sites: Obstacles or Opportunities for Drug Delivery?
Current Pharmaceutical Design Current Treatment Strategies for Multiple Sclerosis - Efficacy Versus Neurological Adverse Effects
Current Pharmaceutical Design Anti-apoptotic Serpins as Therapeutics in Cardiovascular Diseases
Cardiovascular & Hematological Disorders-Drug Targets Role of Hexokinase and VDAC in Neurological Disorders
Current Molecular Pharmacology Endoglin Silencing has Significant Antitumor Effect on Murine Mammary Adenocarcinoma Mediated by Vascular Targeted Effect
Current Gene Therapy Gadolinium-Contrast Toxicity in Patients with Kidney Disease: Nephrotoxicity and Nephrogenic Systemic Fibrosis
Current Drug Safety The Multidrug Resistance Mechanisms and their Interactions with the Radiopharmaceutical Probes Used for an In Vivo Detection
Current Drug Metabolism Pharmacologically Targeting the Primary Defect and Downstream Pathology in Duchenne Muscular Dystrophy
Current Gene Therapy