Abstract
Renal failure due to ischemic injury is a common denominator of various clinical situations in critically ill patients. This study was designed to characterize the TPSO/Cholesterol synthesis and cell division pathways in response to different levels of ischemia. Porcine kidneys were subjected to either 60min-warm ischemia (WI) or auto-transplanted after cold storage for 24h at 4°C (CS), or both conditions (WI+CS), pathway activation and function were evaluated at 3h, 3 and 7 days after reperfusion.
CS combined to WI affects renal functions indicating a high degree of injury. During the first week of reperfusion, renal levels of free and esterified cholesterol, major cellular components, increased in CS group with an attenuated production when WI was associated. CS and WI+CS groups exhibited an elevated expression of cell cycle induction markers such as PCNA and stathmin. TSPO expression was highest in groups with the lowest injury, and correlated with kidney outcome, revealing its potential for diagnosis.
Keywords: Acute kidney injury, hypoxia, ischemia reperfusion injury, repair process
Current Molecular Medicine
Title:Evaluation of Early Regenerative Processes in a Preclinical Pig Model of Acute Kidney Injury
Volume: 12 Issue: 4
Author(s): L. Rossard, F. Favreau, J. Demars, R. Robert, C. Nadeau, J. Cau, R. Thuillier and T. Hauet
Affiliation:
Keywords: Acute kidney injury, hypoxia, ischemia reperfusion injury, repair process
Abstract: Renal failure due to ischemic injury is a common denominator of various clinical situations in critically ill patients. This study was designed to characterize the TPSO/Cholesterol synthesis and cell division pathways in response to different levels of ischemia. Porcine kidneys were subjected to either 60min-warm ischemia (WI) or auto-transplanted after cold storage for 24h at 4°C (CS), or both conditions (WI+CS), pathway activation and function were evaluated at 3h, 3 and 7 days after reperfusion.
CS combined to WI affects renal functions indicating a high degree of injury. During the first week of reperfusion, renal levels of free and esterified cholesterol, major cellular components, increased in CS group with an attenuated production when WI was associated. CS and WI+CS groups exhibited an elevated expression of cell cycle induction markers such as PCNA and stathmin. TSPO expression was highest in groups with the lowest injury, and correlated with kidney outcome, revealing its potential for diagnosis.
Export Options
About this article
Cite this article as:
Rossard L., Favreau F., Demars J., Robert R., Nadeau C., Cau J., Thuillier R. and Hauet T., Evaluation of Early Regenerative Processes in a Preclinical Pig Model of Acute Kidney Injury, Current Molecular Medicine 2012; 12 (4) . https://dx.doi.org/10.2174/1566524011207040502
DOI https://dx.doi.org/10.2174/1566524011207040502 |
Print ISSN 1566-5240 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5666 |
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Matrix Metalloproteinases and their Tissue Inhibitors in Diabetes, Atherosclerosis and Prediction of the Cardiovascular Risk
Current Enzyme Inhibition Modulation of PMN-Endothelial Cells Interactions by Cyclic Nucleotides
Current Pharmaceutical Design Targeting Vascular Disease in Systemic Sclerosis
Endocrine, Metabolic & Immune Disorders - Drug Targets To Die or Not to Die: That is the Autophagic Question
Current Molecular Medicine Desmoteplase for Acute Ischemic Stroke: A Systematic Review and Metaanalysis of Randomized Controlled Trials
CNS & Neurological Disorders - Drug Targets The Discovery of the Factor Xa Inhibitor Otamixaban: From Lead Identification to Clinical Development
Current Medicinal Chemistry The CX3C-Chemokine Fractalkine in Kidney Diseases
Mini-Reviews in Medicinal Chemistry Possibility of Enhanced Risk of Retinal Angiogenesis in Athletes with Pre- Existing Retinal Situation Abusing Erythropoietin Doping: A Hypothesis
Current Drug Safety Effects of Kallistatin on Oxidative Stress and Inflammation on Renal Ischemia-Reperfusion Injury in Mice
Current Vascular Pharmacology The Neuroprotective Mechanism of Erythropoietin-TAT Fusion Protein Against Neurodegeneration from Ischemic Brain Injury
CNS & Neurological Disorders - Drug Targets Patents in Targets and Drugs for Unbalanced Cytokine and Chemokine Network Mediated Disorders
Recent Patents on Inflammation & Allergy Drug Discovery Complications of Anti-Vascular Endothelial Growth Factor Drugs
Current Drug Therapy Endothelin Receptor Antagonists: A New Therapeutic Option for Improving the Outcome after Solid Organ Transplantation?
Current Vascular Pharmacology Clinically Relevant Extracellular-Matrix Scaffolds for Cell Transplantation and Vascular Repair
Current Vascular Pharmacology The Importance of Reendothelialization After Arterial Injury
Current Pharmaceutical Design Role of Endothelial Nitric Oxide in Cerebrovascular Regulation
Current Pharmaceutical Biotechnology Regulation of the PI3K-Akt Network: Current Status and a Promise for the Treatment of Human Diseases
Current Signal Transduction Therapy Association of Dietary Prebiotic Consumption with Reduced Risk of Alzheimer’s Disease in a Multiethnic Population
Current Alzheimer Research Drotrecogin Alfa (Activated) in the Treatment of Severe Sepsis
Current Drug Safety Heparins with Reduced Anti-Coagulant Activity Reduce Myocardial Reperfusion Injury
Recent Patents on Cardiovascular Drug Discovery