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Infectious Disorders - Drug Targets

Editor-in-Chief

ISSN (Print): 1871-5265
ISSN (Online): 2212-3989

Research Article

Sero-epidemiology of Hydatidosis Among General Population of Jolfa County, Northwestern Iran Using IHA, ELISA and Western Blot (2017-2018)

Author(s): Ghorban Sakhaei, Shahram Khademvatan*, Khosrow Hazrati Tappeh, Sima Masudi, Sadegh Feizollahzadeh, Arash Aminpour and Negar Asadi

Volume 21, Issue 2, 2021

Published on: 16 May, 2020

Page: [193 - 201] Pages: 9

DOI: 10.2174/1871526520666200516162813

Price: $65

Abstract

Background: Human hydatidosis is mostly a latent and neglected disease with known endemicity in Iran.

Aims: Due to the importance of this infection in the country and its latent nature, we aimed to evaluate the serological status of hydatid cyst in northwestern Iran.

Objectives: Herein, we evaluated the serological status of hydatid cyst in urban and rural inhabitants of Jolfa county, northwestern Iran during 2017-2018.

Methods: In total, 1296 blood samples were obtained from human individuals and the presence of anti-E. granulosus antibodies was investigated using IHA, ELISA and WB.

Results: Based on results, 25 IHA positive person were detected in the examined population, however ELISA test showed 14 of 25 IHA positive patients as negative. Also, 269 IHA negative fellows were shown as negative by ELISA. WB analysis of sera from 25 IHA positive subjects revealed consistent results with the ELISA test, and the most reactive SHCF Ag was a 37 KDa protein. The age-standardized seroprevalence of hydatidosis among Jolfa's general population was 1.12% with 95%CI: 1.02-1.20. Moreover, there existed a significant association between keeping/- contact with dogs (P = 0.022) as well as vegetable consumption (P < 0.001) with ELISA positive test results.

Conclusion: Along with such serological evidence in this region, we highly suggest physical examination and applying imaging techniques for suspected cases in the area for a better understanding of CE.

Keywords: Seroepidemiology, Human hydatidosis, IHA, ELISA, WB, Iran, Jolfa.

Graphical Abstract
[1]
Torgerson, P.R.; Deplazes, P. Echinococcosis: diagnosis and diagnostic interpretation in population studies. Trends Parasitol., 2009, 25(4), 164-170.
[http://dx.doi.org/10.1016/j.pt.2008.12.008] [PMID: 19269248]
[2]
Torgerson, P.R.; Macpherson, C.N. The socioeconomic burden of parasitic zoonoses: global trends. Vet. Parasitol., 2011, 182(1), 79-95.
[http://dx.doi.org/10.1016/j.vetpar.2011.07.017] [PMID: 21862222]
[3]
Craig, P.S.; McManus, D.P.; Lightowlers, M.W.; Chabalgoity, J.A.; Garcia, H.H.; Gavidia, C.M.; Gilman, R.H.; Gonzalez, A.E.; Lorca, M.; Naquira, C.; Nieto, A.; Schantz, P.M. Prevention and control of cystic echinococcosis. Lancet Infect. Dis., 2007, 7(6), 385-394.
[http://dx.doi.org/10.1016/S1473-3099(07)70134-2] [PMID: 17521591]
[4]
Wen, H.; Vuitton, L.; Tuxun, T.; Li, J.; Vuitton, D.A.; Zhang, W.; McManus, D.P. Echinococcosis: Advances in the 21st Century. Clin. Microbiol. Rev., 2019, 32(2), e00075-e18.
[http://dx.doi.org/10.1128/CMR.00075-18] [PMID: 30760475]
[5]
Budke, C.M.; Carabin, H.; Ndimubanzi, P.C.; Nguyen, H.; Rainwater, E.; Dickey, M.; Bhattarai, R.; Zeziulin, O.; Qian, M-B. A systematic review of the literature on cystic echinococcosis frequency worldwide and its associated clinical manifestations. Am. J. Trop. Med. Hyg., 2013, 88(6), 1011-1027.
[http://dx.doi.org/10.4269/ajtmh.12-0692] [PMID: 23546806]
[6]
Budke, C.M.; Deplazes, P.; Torgerson, P.R. Global socioeconomic impact of cystic echinococcosis. Emerg. Infect. Dis., 2006, 12(2), 296-303.
[http://dx.doi.org/10.3201/eid1202.050499] [PMID: 16494758]
[7]
McManus, D.P.; Gray, D.J.; Zhang, W.; Yang, Y. Diagnosis, treatment, and management of echinococcosis. BMJ, 2012, 344, e3866.
[http://dx.doi.org/10.1136/bmj.e3866] [PMID: 22689886]
[8]
Carmena, D.; Cardona, G.A. Canine echinococcosis: global epidemiology and genotypic diversity. Acta Trop., 2013, 128(3), 441-460.
[http://dx.doi.org/10.1016/j.actatropica.2013.08.002] [PMID: 23954494]
[9]
Otero-Abad, B.; Torgerson, P.R. A systematic review of the epidemiology of echinococcosis in domestic and wild animals. PLoS Negl. Trop. Dis., 2013, 7(6), e2249.
[http://dx.doi.org/10.1371/journal.pntd.0002249] [PMID: 23755310]
[10]
Possenti, A.; Manzano-Román, R.; Sánchez-Ovejero, C.; Boufana, B.; La Torre, G.; Siles-Lucas, M.; Casulli, A. Potential risk factors associated with human cystic echinococcosis: systematic review and meta-analysis. PLoS Negl. Trop. Dis., 2016, 10(11), e0005114.
[http://dx.doi.org/10.1371/journal.pntd.0005114] [PMID: 27820824]
[11]
Alvarez Rojas, C.A.; Romig, T.; Lightowlers, M.W. Echinococcus granulosus sensu lato genotypes infecting humans- review of current knowledge. Int. J. Parasitol., 2014, 44(1), 9-18.
[http://dx.doi.org/10.1016/j.ijpara.2013.08.008] [PMID: 24269720]
[12]
Khademvatan, S.; Yousefi, E.; Rafiei, A.; Rahdar, M.; Saki, J. Molecular characterization of livestock and human isolates of Echinococcus granulosus from south-west Iran. J. Helminthol., 2013, 87(2), 240-244.
[http://dx.doi.org/10.1017/S0022149X12000296] [PMID: 22691442]
[13]
Khalkhali, H.R.; Foroutan, M.; Khademvatan, S.; Majidiani, H.; Aryamand, S.; Khezri, P.; Aminpour, A. Prevalence of cystic echinococcosis in Iran: a systematic review and meta-analysis. J. Helminthol., 2018, 92(3), 260-268.
[http://dx.doi.org/10.1017/S0022149X17000463] [PMID: 28589871]
[14]
Khademvatan, S.; Majidiani, H.; Foroutan, M.; Hazrati Tappeh, K.; Aryamand, S.; Khalkhali, H.R. Echinococcus granulosus genotypes in Iran: a systematic review. J. Helminthol., 2019, 93(2), 131-138.
[http://dx.doi.org/10.1017/S0022149X18000275] [PMID: 29606162]
[15]
Fasihi Harandi, M.; Budke, C.M.; Rostami, S. The monetary burden of cystic echinococcosis in Iran. PLoS Negl. Trop. Dis., 2012, 6(11), e1915.
[http://dx.doi.org/10.1371/journal.pntd.0001915] [PMID: 23209857]
[16]
Eckert, J.; Thompson, R. Historical aspects of echinococcosis. Advances in parasitology. 95; Elsevier, 2017, pp. 1-64.
[17]
Dakkak, A. Echinococcosis/hydatidosis: a severe threat in Mediterranean countries. Vet. Parasitol., 2010, 174(1-2), 2-11.
[http://dx.doi.org/10.1016/j.vetpar.2010.08.009] [PMID: 20888694]
[18]
Grosso, G.; Gruttadauria, S.; Biondi, A.; Marventano, S.; Mistretta, A. Worldwide epidemiology of liver hydatidosis including the Mediterranean area. World J. Gastroenterol., 2012, 18(13), 1425-1437.
[http://dx.doi.org/10.3748/wjg.v18.i13.1425] [PMID: 22509074]
[19]
Moro, P.; Schantz, P.M. Echinococcosis: a review. Int. J. Infect. Dis., 2009, 13(2), 125-133.
[http://dx.doi.org/10.1016/j.ijid.2008.03.037] [PMID: 18938096]
[20]
Brunetti, E.; Kern, P.; Vuitton, D.A. Writing Panel for the WHO-IWGE. Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop., 2010, 114(1), 1-16.
[http://dx.doi.org/10.1016/j.actatropica.2009.11.001] [PMID: 19931502]
[21]
Sarkari, B.; Rezaei, Z. Immunodiagnosis of human hydatid disease: Where do we stand? World J. Methodol., 2015, 5(4), 185-195.
[http://dx.doi.org/10.5662/wjm.v5.i4.185] [PMID: 26713278]
[22]
Arbabi, M.; Hooshyar, H. Survey of echinococcosis and hydatidosis in Kashan region, central Iran. Iran. J. Public Health, 2006, 75-81.
[23]
Abo-Shehada, M.N. Some observations on hydatidosis in Jordan. J. Helminthol., 1993, 67(3), 248-252.
[http://dx.doi.org/10.1017/S0022149X00013201] [PMID: 8288858]
[24]
Dyab, K.A.; Hassanein, R.; Hussein, A.A.; Metwally, S.E.; Gaad, H.M. Hydatidosis among man and animals in Assiut and Aswan Governorates. J. Egypt. Soc. Parasitol., 2005, 35(1), 157-166.
[PMID: 15881003]
[25]
Cetinkaya, Z.; Ciftci, I.H.; Demirel, R.; Altindis, M.; Ayaz, E. A sero-epidemiologic study on cystic echinococcosis in midwestern region of Turkey. Saudi Med. J., 2005, 26(2), 350-351.
[PMID: 15770329]
[26]
Eşgin, M; Aktaş, M; Coşkun, S The investigation of antibody presence in the sera of patients with a suspicion of cystic echinococcosis by using indirect hemaglutination test (IHA). Turkiye parazitolojii dergisi., 2007, 31(4), 283-287..
[27]
Güreser, A.S.; Özcan, O.; Özünel, L.; Boyacıoğlu, Z.İ.; Taylan Özkan, A. Evaluation of the radiological, biochemical and serological parameters of patients prediagnosed as cystic echinococcosis in Çorum, Turkey. Mikrobiyol. Bul., 2015, 49(2), 231-239.
[http://dx.doi.org/10.5578/mb.8656] [PMID: 26167823]
[28]
Karaman, U; Mıman, O; Kara, M; Gicik, Y; Aycan, O; Atambay, M Hydatid cyst prevalence in the region of Kars. Turkiye parazitolojii dergisi., 2005, 29(4), 238-240..
[29]
Yazıcı, V.; Oruç, T.; Oren, E.; Ertabaklar, H. Retrospective evaluation of patients with probable cystic echinococcosis to the central laboratory of the Kocaeli Derince education and research hospital between 2009 and 2011. Turkiye Parazitol. Derg., 2012, 36(4), 219-221.
[http://dx.doi.org/10.5152/tpd.2012.53] [PMID: 23339943]
[30]
Haghpanah, B.; Mosavat, B.; Ghayour, Z.; Oreizi, F. Diagnostic value of hydatid cyst antigens using western blotting method. Jundishapur J. Microbiol., 2010, 3(4), 175.
[31]
Kilimcioğlu, A.A.; Girginkardeşler, N.; Korkmaz, M.; Özkol, M.; Düzgün, F.; Östan, I.; Pabuşcu, Y.; Dinç, G.; Ok, Ü.Z. A mass screening survey of cystic echinococcosis by ultrasonography, Western blotting, and ELISA among university students in Manisa, Turkey. Acta Trop., 2013, 128(3), 578-583.
[http://dx.doi.org/10.1016/j.actatropica.2013.08.010] [PMID: 23978681]
[32]
Galeh, T.M.; Spotin, A.; Mahami-Oskouei, M.; Carmena, D.; Rahimi, M.T.; Barac, A.; Ghoyounchi, R.; Berahmat, R.; Ahmadpour, E. The seroprevalence rate and population genetic structure of human cystic echinococcosis in the Middle East: A systematic review and meta-analysis. Int. J. Surg., 2018, 51, 39-48.
[http://dx.doi.org/10.1016/j.ijsu.2018.01.025] [PMID: 29367032]
[33]
Acosta-Jamett, G.; Weitzel, T.; Boufana, B.; Adones, C.; Bahamonde, A.; Abarca, K.; Craig, P.S.; Reiter-Owona, I. Prevalence and risk factors for echinococcal infection in a rural area of northern Chile: a household-based cross-sectional study. PLoS Negl. Trop. Dis., 2014, 8(8), e3090.
[http://dx.doi.org/10.1371/journal.pntd.0003090] [PMID: 25167140]
[34]
Logar, J.; Soba, B.; Kotar, T. Serological evidence for human cystic echinococcosis in Slovenia. BMC Infect. Dis., 2008, 8(1), 63.
[http://dx.doi.org/10.1186/1471-2334-8-63] [PMID: 18471283]
[35]
Doiz, O.; Benito, R.; Sbihi, Y.; Osuna, A.; Clavel, A.; Gómez-Lus, R. Western blot applied to the diagnosis and post-treatment monitoring of human hydatidosis. Diagn. Microbiol. Infect. Dis., 2001, 41(3), 139-142.
[http://dx.doi.org/10.1016/S0732-8893(01)00293-0] [PMID: 11750167]
[36]
Sbihi, Y.; Janssen, D.; Osuna, A. Serologic recognition of hydatid cyst antigens using different purification methods. Diagn. Microbiol. Infect. Dis., 1996, 24(4), 205-211.
[http://dx.doi.org/10.1016/0732-8893(96)00061-2] [PMID: 8831035]
[37]
Fomda, B.A.; Khan, A.; Thokar, M.A.; Malik, A.A.; Fazili, A.; Dar, R.A.; Sharma, M.; Malla, N. Sero-epidemiological survey of human cystic echinococcosis in Kashmir, North India. PLoS One, 2015, 10(4), e0124813.
[http://dx.doi.org/10.1371/journal.pone.0124813] [PMID: 25915867]
[38]
Heidari, Z.; Mohebali, M.; Zarei, Z.; Aryayipour, M.; Eshraghian, M.; Kia, E.; Shodajei, S.; Abdi, J.; Rakhshanpour, A.; Rokni, M. Seroepidemiological study of human hydatidosis in meshkinshahr district, ardabil province, iran. Iran. J. Parasitol., 2011, 6(3), 19-25.
[PMID: 22347293]
[39]
Craig, P.S.; Budke, C.M.; Schantz, P.M.; Li, T.; Qiu, J.; Yang, Y.; Zeyhle, E.; Rogan, M.T.; Ito, A. Human echinococcosis: a neglected disease? Trop. Med. Health, 2007, 35(4), 283-292.
[http://dx.doi.org/10.2149/tmh.35.283]
[40]
Yang, Y.R.; Craig, P.S.; Vuitton, D.A.; Williams, G.M.; Sun, T.; Liu, T.X.; Boufana, B.; Giraudoux, P.; Teng, J.; Li, Y.; Huang, L.; Zhang, W.; Jones, M.K.; McManus, D.P. Serological prevalence of echinococcosis and risk factors for infection among children in rural communities of southern Ningxia, China. Trop. Med. Int. Health, 2008, 13(8), 1086-1094.
[http://dx.doi.org/10.1111/j.1365-3156.2008.02101.x] [PMID: 18554249]

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