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Current Pharmaceutical Design


ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

General Research Article

Expression of Rab3b in Human Glioma: Influence on Cell Proliferation and Apoptosis

Author(s): Qili Luo, Yueping Liu, Zilin Yuan, Lvshuai Huang* and Bo Diao*

Volume 27, Issue 7, 2021

Published on: 17 September, 2020

Page: [989 - 995] Pages: 7

DOI: 10.2174/1381612826666200917145228

Price: $65


Background: Glioma is the most common human central nervous system tumour with a high degree of malignancy. Some Rab GTPases have significant effects on glioma.

Objective: This study aimed to investigate the effect of Rab3b (Rab GTPase3b) on human glioma cell proliferation and apoptosis by silencing Rab3b and to initially verify the value of Rab3b expression for the diagnosis and progression in human glioma.

Methods: Rab3b was silenced by siRNA transfection. Human glioma tissues and normal brain tissues adjacent to glioma were obtained by surgery. Rab3b, P53, Caspase 7, Bax, and Bim mRNA and protein expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. Cell proliferation was detected by the cell counting kit-8 assay, and the cell cycle and apoptosis were analysed using flow cytometry.

Results: Rab3b mRNA and protein expression in human glioma U251 and U87 cells were significantly downregulated after Rab3b silencing. Rab3b silencing inhibited glioma cell proliferation by promoting cell cycle arrest and induced apoptosis by upregulating the expression of apoptosis-related proteins. Rab3b expression in human glioma (n = 33) was significantly higher than that in normal brain tissues adjacent to glioma (n = 15). In addition, Rab3b expression levels in high-grade gliomas (WHO III-IV, n = 19) were also significantly higher than those in low-grade gliomas (WHO I-II, n = 14).

Conclusion: Rab3b expression levels are significantly related to the progression of gliomas. Moreover, Rab3b silencing not only significantly inhibits cell proliferation in gliomas via cell cycle arrest but also promotes cell apoptosis by upregulating the expression levels of apoptosis-related proteins; however these preliminary in vitro results warrant validation on in vivo studies.

Keywords: Rab3b, human glioma, apoptosis, proliferation, diagnosis, progression.

« Previous
Zhao X, Chen R, Liu M, Feng J, Chen J, Hu K. Remodeling the blood-brain barrier microenvironment by natural products for brain tumor therapy. Acta Pharm Sin B 2017; 7(5): 541-53.
[] [PMID: 28924548]
Hutagalung AH, Novick PJ. Role of Rab GTPases in membrane traffic and cell physiology. Physiol Rev 2011; 91(1): 119-49.
[] [PMID: 21248164]
Guadagno NA, Progida C. Rab GTPases: Switching to human diseases. Cells 2019; 8(8): 909.
[] [PMID: 31426400]
Ge J, Chen Q, Liu B, Wang L, Zhang S, Ji B. Knockdown of Rab21 inhibits proliferation and induces apoptosis in human glioma cells. Cell Mol Biol Lett 2017; 22(1): 30.
[] [PMID: 29270202]
Wang HJ, Gao Y, Chen L, Li YL, Jiang CL. RAB34 was a progression- and prognosis-associated biomarker in gliomas. Tumour Biol 2015; 36(3): 1573-8.
[] [PMID: 25501506]
Ding B, Cui B, Gao M, et al. Knockdown of Ras-related protein 25 (rab25) inhibits the in vitro cytotoxicity and in vivo antitumor activity of human glioblastoma multiforme cells. Oncol Res 2017; 25(3): 331-40.
[] [PMID: 28281975]
Diao B, Huang XY, Yang CY, et al. Rab13 silencing causes inhibition of growth and induction of appotosis in human glioma cells. Int J Clin Exp Pathol 2016; 9(3): 3007-14.
Starkweather AR, Sherwood P, Lyon DE, McCain NL, Bovbjerg DH, Broaddus WC. A biobehavioral perspective on depressive symptoms in patients with cerebral astrocytoma. J Neurosci Nurs 2011; 43(1): 17-28.
[] [PMID: 21338041]
Mahmoudi K, Ezrin A, Hadjipanayis C. Small extracellular vesicles as tumor biomarkers for glioblastoma. Mol Aspects Med 2015; 45: 97-102.
[] [PMID: 26118341]
Tan PY, Chang CW, Chng KR, Wansa KDSA, Sung WK, Cheung E. Integration of regulatory networks by NKX3-1 promotes androgen-dependent prostate cancer survival. Mol Cell Biol 2012; 32(2): 399-414.
[] [PMID: 22083957]
Liu Q, Tang H, Liu X, et al. miR-200b as a prognostic factor targets multiple members of RAB family in glioma. Med Oncol 2014; 31(3): 859.
[] [PMID: 24477653]
Kim JK, Lee SY, Park CW, et al. Rab3a promotes brain tumor initiation and progression. Mol Biol Rep 2014; 41(9): 5903-11.
[] [PMID: 24965146]
Chen J. The Cell-Cycle Arrest and Apoptotic Functions of p53 in Tumor Initiation and Progression. Cold Spring Harb Perspect Med 2016; 6(3): a026104.
[] [PMID: 26931810]
Yang SH, Wang SM, Syu JP, et al. Andrographolide induces apoptosis of C6 glioma cells via the ERK-p53-caspase 7-PARP pathway. BioMed Res Int 2014; 2014: 312847.
[] [PMID: 25162007]
Zhao L, Feng Y, Shi A, Zong Y, Wan M. Apoptosis induced by microbubble-assisted acoustic cavitation in K562 Cells: The predominant role of the cyclosporin a-dependent mitochondrial permeability transition pore. Ultrasound Med Biol 2015; 41(10): 2755-64.
[] [PMID: 26164288]

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