Abstract
Characterization of carbohydrate moieties of glycoproteins using microwave-assisted partial acid hydrolysis (MAPAH) and mass spectrometry (MS) are described in this review. Acids including hydrochloric acid (HCl), trifluoroacetic acid (TFA), and phosphoric acid (H3PO4) can be used to induce partial hydrolysis of the carbohydrate moieties of glycoproteins in as short as 30 s of microwave exposure. High resolving power of MS allows distinction between different glycopeptide fragments. Several glycoproteins including bovine pancreatic ribonuclease B (RNase B, MW ∼15 kDa), egg white avidin (MW ∼16 kDa), human serum α1-acid glycoprotein (α1-AGP, MW ∼36 kDa), horseradish peroxidase (HRP, MW ∼44 kDa), fetal calf serum fetuin (BSF, MW ∼45 kDa), and glucose oxidase from Aspergillus niger (GO, MW ∼75 kDa), are used to demonstrate this technique. Information of the sugar composition and/or arrangement of the carbohydrate moieties can be readily obtained either from glycoproteins directly or digested glycopeptides of the glycoproteins. This method is fast, sensitive, and a reliable technique. Challenges when working on larger and heavily glycosylated glycoproteins are discussed.
Keywords: Glycoproteins, Glycopeptides, Carbohydrate, Posttranslational modification (PTM), Microwave-assisted partial, acid hydrolysis (MAPAH), Glasstip static probe, Nanospray, MALDI-TOF MS, FT-ICR MS
Current Analytical Chemistry
Title: Structural Analyses of Carbohydrate Moieties of Glycoproteins by Microwave-Assisted Partial Acid Hydrolysis and Mass Spectrometry
Volume: 4 Issue: 1
Author(s): Bao-Shiang Lee, Sangeeth Krishnanchettiar, G. D. Lasanthi P. Jayathilaka, Syed Salman Lateef and Shalini Gupta
Affiliation:
Keywords: Glycoproteins, Glycopeptides, Carbohydrate, Posttranslational modification (PTM), Microwave-assisted partial, acid hydrolysis (MAPAH), Glasstip static probe, Nanospray, MALDI-TOF MS, FT-ICR MS
Abstract: Characterization of carbohydrate moieties of glycoproteins using microwave-assisted partial acid hydrolysis (MAPAH) and mass spectrometry (MS) are described in this review. Acids including hydrochloric acid (HCl), trifluoroacetic acid (TFA), and phosphoric acid (H3PO4) can be used to induce partial hydrolysis of the carbohydrate moieties of glycoproteins in as short as 30 s of microwave exposure. High resolving power of MS allows distinction between different glycopeptide fragments. Several glycoproteins including bovine pancreatic ribonuclease B (RNase B, MW ∼15 kDa), egg white avidin (MW ∼16 kDa), human serum α1-acid glycoprotein (α1-AGP, MW ∼36 kDa), horseradish peroxidase (HRP, MW ∼44 kDa), fetal calf serum fetuin (BSF, MW ∼45 kDa), and glucose oxidase from Aspergillus niger (GO, MW ∼75 kDa), are used to demonstrate this technique. Information of the sugar composition and/or arrangement of the carbohydrate moieties can be readily obtained either from glycoproteins directly or digested glycopeptides of the glycoproteins. This method is fast, sensitive, and a reliable technique. Challenges when working on larger and heavily glycosylated glycoproteins are discussed.
Export Options
About this article
Cite this article as:
Lee Bao-Shiang, Krishnanchettiar Sangeeth, Lasanthi P. Jayathilaka D. G., Lateef Salman Syed and Gupta Shalini, Structural Analyses of Carbohydrate Moieties of Glycoproteins by Microwave-Assisted Partial Acid Hydrolysis and Mass Spectrometry, Current Analytical Chemistry 2008; 4 (1) . https://dx.doi.org/10.2174/157341108783339089
DOI https://dx.doi.org/10.2174/157341108783339089 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
Call for Papers in Thematic Issues
Advancements in biomedicine, corrosion inhibition/monitoring, and lubrication of carbon dots
Carbon dots, a class of carbon-based nanomaterials, have garnered substantial interest in various scientific and engineering fields due to their unique properties, including excellent optical properties, outstanding biocompatibility, easy preparation, good dispersity, ultrasmall size, low toxicity and environmental friendliness. This special issue of Current Analytical Chemistry aims to showcase cutting-edge ...read more
Advances in Microfluidic and Sensing Technologies of Biofluids.
Detection of biological fluids is an important means to assess human health and diagnose diseases. Currently, research on blood is relatively mature and widely applied in the medical field. With the development and advancement of microfluidic technology and novel detection methods, attention has turned to human physiological fluids such as ...read more
Analytical methods for environmental and food analysis
In recent years, the study of the chemical composition of food and the environment depends on analytical methods in order to acquire information about the chemical composition, processing, quality control and contamination of soil, water, air, plants and foods, aspects very important for food safety and environmental quality control. There ...read more
Applications of artificial intelligence in chemical analysis
Artificial intelligence (AI) has demonstrated its versatility in almost all scientific fields, especially analytical chemistry, which is leading the way in the integration of AI. According to Chemical Abstract Services, one of the major disciplines contributing to AI-related publications and patents is analytical chemistry. That?s because techniques such as deep ...read more
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
Related Articles
-
Biodistribution, Safety and Organ Toxicity of Docetaxel-Loaded in HER-2 Aptamer Conjugated Ecoflex® Nanoparticles in a Mouse Xenograft Model of Ovarian Cancer
Recent Patents on Nanotechnology Editorial [Hot Topic:Applications of Angiotensin Converting Enzyme Inhibitors and of Angiotensin II Receptor Blockers in Pharmacology and Therapy: An Update (Executive Editor: Agostino Molteni)]
Current Pharmaceutical Design Vitamin Bs, One Carbon Metabolism and Prostate Cancer
Mini-Reviews in Medicinal Chemistry ACKNOWLEDGEMENTS TO REVIEWERS
Endocrine, Metabolic & Immune Disorders - Drug Targets Neuropsychiatric Aspects in Men with Klinefelter Syndrome
Endocrine, Metabolic & Immune Disorders - Drug Targets The Chemopreventive Peptide Lunasin Inhibits d-Galactose- Induced Experimental Cataract in Rats
Protein & Peptide Letters The Oncogenic Functions of Insulin-like Growth Factor 2 mRNA-Binding Protein 3 in Human Carcinomas
Current Pharmaceutical Design Myokines in Myogenesis and Health
Recent Patents on Biotechnology Non Smoking for Successful Aging: Therapeutic Perspectives
Current Pharmaceutical Design Lipid Transport into Bile and Role in Bile Formation
Current Drug Targets - Immune, Endocrine & Metabolic Disorders Neuroprotection and Hypothermia in Infants and Children
Current Drug Targets Therapeutic Potential of Coagonists of Glucagon and GLP-1
Cardiovascular & Hematological Agents in Medicinal Chemistry Towards Standardized Stem Cell Therapy in Type 2 Diabetes Mellitus: A Systematic Review
Current Stem Cell Research & Therapy Indicators of Obesity and Cardio-metabolic Risks: Important Consideration in Adults and Children
Current Diabetes Reviews Physiology and Pharmacology of Two-Pore Domain Potassium Channels
Current Pharmaceutical Design Spray Dried Plasma for Pigs Weaned at Different Ages
Recent Patents on Food, Nutrition & Agriculture Emerging Immunosuppressive Drugs in Kidney Transplantation
Current Clinical Pharmacology Editorial
Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents Tankyrase as a Novel Molecular Target in Cancer and Fibrotic Diseases
Current Drug Targets Transcriptional Regulation as a Pharmacologic Intervention
Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents