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Protein & Peptide Letters


ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Research Article

ACE2 Receptor Polymorphism and its Correlation with Anti-SARS-CoV-2 IgM Antibodies - A Case-Control Study

Author(s): Anum Siraj, Saira Yahya*, Amber Khan and Saleem Awan

Volume 30, Issue 2, 2023

Published on: 30 December, 2022

Page: [146 - 153] Pages: 8

DOI: 10.2174/0929866530666221206094926

Price: $65


Background: The SARS-CoV-2 pandemic originated in Wuhan, China in December 2019 and spread rapidly worldwide. The virus gets entry into target cells via angiotensin-converting enzyme 2 (ACE2) receptors and its gene is highly polymorphic.

Introduction: The variations in SARS-CoV-2 susceptibility and severity can be explained on a genetic level by studying the polymorphism in ACE2 receptor polymorphism.

Objective: A prospective case-control study was designed to compare the ACE2 levels in SARS-CoV- 2 patients with the healthy controls in the local population, for which a total of 100 EDTA-containing blood samples were included (50 SARS-CoV-2 IgM positive case and 50 healthy controls).

Methods: PCR-RFLP was performed to investigate the polymorphism of ACE2 in genomic DNA and the ACE2 plasma levels were determined through ELISA.

Results: No significant difference in allelic and genotype frequencies (GG, GA, AA) were observed while the ACE2 plasma levels were found to be decreased in positive samples.

Conclusion: No significant association of the ACE2 gene polymorphism (G8790A) was found with the SARS-CoV-2 susceptibility in the Pakistani population which intimates the search for other genetic factors within the local population.

Keywords: Angiotensin-converting enzyme 2, severe acute respiratory syndrome coronavirus 2, renin-angiotensin system, restriction fragment length polymorphism, enzyme-linked immunosorbent assay.

Graphical Abstract
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